Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 949034..949605 Replicon chromosome
Accession NZ_CP038163
Organism Enterococcus faecium strain GZ8

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag E3O43_RS04815 Protein ID WP_002286801.1
Coordinates 949034..949375 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag E3O43_RS04820 Protein ID WP_002323011.1
Coordinates 949375..949605 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3O43_RS04790 (E3O43_05030) 944244..945578 - 1335 WP_283651788.1 ABC transporter substrate-binding protein -
E3O43_RS04795 (E3O43_05035) 945786..946616 + 831 WP_002286789.1 manganese catalase family protein -
E3O43_RS04800 (E3O43_05045) 946860..947109 - 250 Protein_911 transposase -
E3O43_RS04805 (E3O43_05050) 947300..947494 - 195 WP_002295146.1 hypothetical protein -
E3O43_RS04810 (E3O43_05055) 947999..948184 - 186 WP_002304207.1 hypothetical protein -
E3O43_RS04815 (E3O43_05060) 949034..949375 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E3O43_RS04820 (E3O43_05065) 949375..949605 - 231 WP_002323011.1 hypothetical protein Antitoxin
E3O43_RS04825 (E3O43_05075) 949892..950230 + 339 WP_002286804.1 hypothetical protein -
E3O43_RS04830 (E3O43_05080) 950435..951010 - 576 WP_002293673.1 SOS response-associated peptidase family protein -
E3O43_RS04835 (E3O43_05095) 951619..952200 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
E3O43_RS04840 (E3O43_05100) 952383..953009 - 627 WP_002333420.1 cysteine hydrolase -
E3O43_RS04845 (E3O43_05105) 953031..954362 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T120732 WP_002286801.1 NZ_CP038163:c949375-949034 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T120732 NZ_CP038163:c949375-949034 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT120732 WP_002323011.1 NZ_CP038163:c949605-949375 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT120732 NZ_CP038163:c949605-949375 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References