Detailed information of TA system TA120729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2680165..2680816
• Replicon: chromosome
• Accession: NZ_CP038153
• Organism: Lacticaseibacillus paracasei strain TCS

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K6S8L5
• Locus tag: E3E17_RS14145
• Protein ID: WP_003567661.1
• Coordinates: 2680165..2680548 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K0NCT7
• Locus tag: E3E17_RS14150
• Protein ID: WP_014566665.1
• Coordinates: 2680568..2680816 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3E17_RS14140	2679470..2679991	-	522	WP_012492007.1	QueT transporter family protein	-
E3E17_RS14145	2680165..2680548	-	384	WP_003567661.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3E17_RS14150	2680568..2680816	-	249	WP_014566665.1	antitoxin	Antitoxin
E3E17_RS14155	2680884..2682020	-	1137	WP_003576478.1	alanine racemase	-
E3E17_RS14160	2682007..2682381	-	375	WP_003567669.1	holo-ACP synthase	-
E3E17_RS14165	2682551..2684059	-	1509	WP_003567670.1	DEAD/DEAH box helicase	-
E3E17_RS14170	2684359..2685747	-	1389	WP_003567677.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13773.03 Da
• Isoelectric Point: 10.9764

>T120729 WP_003567661.1 NZ_CP038153:c2680548-2680165 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T120729 NZ_CP038153:c2680548-2680165 [Lacticaseibacillus paracasei]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8966.14 Da
• Isoelectric Point: 4.4610

>AT120729 WP_014566665.1 NZ_CP038153:c2680816-2680568 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEVYAH
IR

Nucleotide

• Length: 249 bp

>AT120729 NZ_CP038153:c2680816-2680568 [Lacticaseibacillus paracasei]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGTATACGCCCAT
ATTCGATGA

Structures

Toxin

• AlphaFold DB: K6S8L5

Antitoxin

• AlphaFold DB: K0NCT7