Detailed information of TA system TA120727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 462491..463119
• Replicon: chromosome
• Accession: NZ_CP038153
• Organism: Lacticaseibacillus paracasei strain TCS

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A806L3G4
• Locus tag: E3E17_RS02395
• Protein ID: WP_012490993.1
• Coordinates: 462751..463119 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: E3E17_RS02390
• Protein ID: WP_003583264.1
• Coordinates: 462491..462751 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3E17_RS02365	458382..459125	+	744	WP_012490990.1	SDR family oxidoreductase	-
E3E17_RS02370	459238..459864	+	627	WP_003577713.1	flavodoxin	-
E3E17_RS02375	460115..460618	+	504	WP_012490991.1	TetR/AcrR family transcriptional regulator	-
E3E17_RS02380	460623..461684	+	1062	WP_003563374.1	ABC transporter permease	-
E3E17_RS02385	461689..462378	+	690	WP_012490992.1	ABC transporter ATP-binding protein	-
E3E17_RS02390	462491..462751	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
E3E17_RS02395	462751..463119	-	369	WP_012490993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3E17_RS02400	463410..464780	-	1371	WP_012490994.1	FAD-dependent oxidoreductase	-
E3E17_RS02405	465144..465962	-	819	WP_012490995.1	Cof-type HAD-IIB family hydrolase	-
E3E17_RS02410	466278..466547	-	270	WP_012490996.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
E3E17_RS02415	466788..467795	+	1008	WP_003563383.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14970.02 Da
• Isoelectric Point: 8.3986

>T120727 WP_012490993.1 NZ_CP038153:c463119-462751 [Lacticaseibacillus paracasei]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMQERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQSNLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T120727 NZ_CP038153:c463119-462751 [Lacticaseibacillus paracasei]
ATGTATAAAATTGACTTCTATGAGGATCGCGATGGCTACTCTGAGATCGAAGACTTTCTTGATCAGTTACGCCATAGTCA
TCAAAAAAATAATGTGAGCCTTTTAAACAAAATCACAAAGGAACTCTACATGCTGCAAAATCTTGGTCCGCGCTTACGTG
AACCTCACGCGAAGTTTCTGAAGGGATATGCTTATCCGATCATGGAATTGCGTCCCATGCAAGAAAGAATCTTTTATGCT
GCATGGCAAAAGGATCATTTCGTTTTGCTTCATCACTACACCAAGAAAACAAACAAAACTGATCACAGAGAAATTTTACG
GGCACAGTCTAACCTCGAAGATTGGCTCTCTAGAAAGGAAGAAAATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT120727 WP_003583264.1 NZ_CP038153:c462751-462491 [Lacticaseibacillus paracasei]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT120727 NZ_CP038153:c462751-462491 [Lacticaseibacillus paracasei]
ATGACGACATGGGAAGACTTCAGTCAGAAACTGAAGAAGGACAATCCAGAAAAGTTGGCAATCATCAAAACCTTGGCCGA
TTTAGAAAGTGAACGAATCAAGCAAGGAATCTCTCAAAAGGACTTTGCAGAAAAAATTGGTATGAAACAGCCGCAACTAG
CAAAGATTGAGCGCTTAGACTCTCTTCCGACTCTGGCAACACTCAATCGATACGCGAGAGGGCTTGGAAAAGAAATTCGT
TTATCAATTGTTACTCAGTGA

Structures

Toxin

• AlphaFold DB: A0A806L3G4