Detailed information of TA system TA120722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 20504..21101
• Replicon: plasmid unnamed1
• Accession: NZ_CP038142
• Organism: Swingsia samuiensis strain AH83

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: E3D00_RS10445
• Protein ID: WP_141462495.1
• Coordinates: 20796..21101 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: E3D00_RS10440
• Protein ID: WP_141462493.1
• Coordinates: 20504..20803 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3D00_RS10405	16182..16673	+	492	WP_141462487.1	helix-turn-helix domain-containing protein	-
E3D00_RS10415	16976..17608	+	633	Protein_21	IS6 family transposase	-
E3D00_RS10420	17759..18391	+	633	WP_141462488.1	hypothetical protein	-
E3D00_RS10425	18477..18668	+	192	WP_141462489.1	hypothetical protein	-
E3D00_RS10430	18697..19401	-	705	WP_141462490.1	IS6 family transposase	-
E3D00_RS10435	19815..20387	-	573	WP_141462491.1	recombinase family protein	-
E3D00_RS10440	20504..20803	-	300	WP_141462493.1	putative addiction module antidote protein	Antitoxin
E3D00_RS10445	20796..21101	-	306	WP_141462495.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3D00_RS10450	21419..22000	-	582	Protein_28	IS6 family transposase	-
E3D00_RS10455	22421..23677	-	1257	WP_141462497.1	Y-family DNA polymerase	-
E3D00_RS10460	23677..24057	-	381	WP_141462498.1	S24 family peptidase	-
E3D00_RS10465	24180..24758	+	579	Protein_31	IS6 family transposase	-
E3D00_RS10470	25165..25533	-	369	WP_141462500.1	hypothetical protein	-
E3D00_RS10575	25695..25835	-	141	WP_181442025.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30193	30193
	-	flank	IS/Tn	-	-	17015..17608	593
	-	flank	IS/Tn	-	-	24180..24329	149

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (3-92)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11396.24 Da
• Isoelectric Point: 10.7710

>T120722 WP_141462495.1 NZ_CP038142:c21101-20796 [Swingsia samuiensis]
MIELKQTSTFTKWESRLRDKRAKTMIAARLMRLAAGLPGDVKSVGEGVSELRIHYGPGYRVYFQQRGSVVIILLCGGDKG
SQSKDIMTAKHLARKWSERND

Nucleotide

• Length: 306 bp

>T120722 NZ_CP038142:c21101-20796 [Swingsia samuiensis]
ATGATTGAACTGAAGCAGACATCAACTTTTACCAAATGGGAATCTCGTCTACGGGATAAGCGAGCAAAAACGATGATTGC
AGCACGACTGATGCGTCTCGCTGCAGGTTTGCCTGGAGATGTAAAATCTGTTGGTGAGGGTGTGAGTGAACTGCGTATCC
ATTACGGACCGGGATACCGTGTTTATTTTCAGCAACGTGGTTCTGTTGTTATTATTTTGCTGTGCGGTGGCGATAAAGGT
TCACAATCAAAAGACATCATGACCGCAAAGCACTTGGCGCGTAAATGGAGTGAACGAAATGACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10637.07 Da
• Isoelectric Point: 4.7962

>AT120722 WP_141462493.1 NZ_CP038142:c20803-20504 [Swingsia samuiensis]
MTEKLTNYDPASALVDDEEIAAFMADAFETADAAYIAKALGVVARAKGMSEIARQTGLSREQLYRSFSERGNPTLKTTLA
VMRALGVDITAKVHQDAAV

Nucleotide

• Length: 300 bp

>AT120722 NZ_CP038142:c20803-20504 [Swingsia samuiensis]
ATGACTGAGAAACTAACAAATTACGATCCAGCCTCTGCTCTTGTCGATGATGAAGAAATCGCAGCCTTTATGGCGGATGC
CTTTGAAACGGCAGATGCGGCTTATATCGCGAAAGCTTTAGGGGTCGTGGCTCGTGCCAAGGGTATGAGTGAGATTGCGC
GACAGACGGGTCTGTCACGTGAACAGCTCTATCGTTCGTTTAGTGAGCGTGGAAACCCAACATTGAAAACAACACTCGCT
GTTATGCGCGCTTTAGGCGTGGATATAACAGCCAAGGTCCATCAAGACGCGGCTGTCTAG