Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 94432..94954 Replicon plasmid pG3X16-2-3
Accession NZ_CP038140
Organism Escherichia coli strain G3X16-2

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag E3N82_RS25030 Protein ID WP_000220560.1
Coordinates 94673..94954 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag E3N82_RS25025 Protein ID WP_000121743.1
Coordinates 94432..94683 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3N82_RS24990 90103..90384 + 282 WP_001185482.1 hypothetical protein -
E3N82_RS24995 90409..91386 + 978 WP_024245156.1 hypothetical protein -
E3N82_RS25000 91383..91808 - 426 WP_000202942.1 hypothetical protein -
E3N82_RS25005 91876..92322 - 447 Protein_102 hypothetical protein -
E3N82_RS25010 92362..93198 - 837 WP_001050931.1 replication initiation protein -
E3N82_RS25025 94432..94683 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
E3N82_RS25030 94673..94954 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3N82_RS25035 95029..95726 + 698 Protein_106 IS1 family transposase -
E3N82_RS25040 95736..96044 + 309 Protein_107 RepB family plasmid replication initiator protein -
E3N82_RS25055 97336..98304 + 969 WP_030005799.1 IS5 family transposase -
E3N82_RS25065 98444..98830 - 387 WP_001039464.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid erm(42) / tet(X4) / floR / blaTEM-1B / tet(M) / tet(A) / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(6)-Id / aph(3'')-Ib - 1..138950 138950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T120720 WP_000220560.1 NZ_CP038140:94673-94954 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T120720 NZ_CP038140:94673-94954 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT120720 WP_000121743.1 NZ_CP038140:94432-94683 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT120720 NZ_CP038140:94432-94683 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References