Detailed information of TA system TA120719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 7097..7644
• Replicon: plasmid pG3X16-2-3
• Accession: NZ_CP038140
• Organism: Escherichia coli strain G3X16-2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: E3N82_RS24485
• Protein ID: WP_050858704.1
• Coordinates: 7363..7644 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A829FT18
• Locus tag: E3N82_RS24480
• Protein ID: WP_000079938.1
• Coordinates: 7097..7366 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3N82_RS24430	2340..2756	-	417	WP_033548869.1	hypothetical protein	-
E3N82_RS24435	3341..3676	+	336	WP_001039462.1	hypothetical protein	-
E3N82_RS24440	3685..3876	+	192	WP_001515192.1	hypothetical protein	-
E3N82_RS24445	3887..4470	-	584	Protein_5	IS1 family transposase	-
E3N82_RS24450	5004..5759	+	756	WP_000807690.1	replication initiation protein RepE	-
E3N82_RS24460	6028..6373	+	346	Protein_7	IS1 family transposase	-
E3N82_RS25430	6884..7024	+	141	WP_160784086.1	hypothetical protein	-
E3N82_RS24480	7097..7366	+	270	WP_000079938.1	hypothetical protein	Antitoxin
E3N82_RS24485	7363..7644	+	282	WP_050858704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3N82_RS25435	7697..7834	+	138	WP_152931508.1	hypothetical protein	-
E3N82_RS24495	8172..8429	-	258	WP_063072981.1	biofilm development YmgB/AriR family protein	-
E3N82_RS24500	8769..8873	-	105	Protein_13	integrase core domain-containing protein	-
E3N82_RS24505	9068..9493	-	426	WP_063072980.1	ester cyclase	-
E3N82_RS24510	9502..10035	-	534	Protein_15	helix-turn-helix domain-containing protein	-
E3N82_RS24515	10070..11990	-	1921	Protein_16	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	erm(42) / tet(X4) / floR / blaTEM-1B / tet(M) / tet(A) / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(6)-Id / aph(3'')-Ib	-	1..138950	138950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10860.58 Da
• Isoelectric Point: 8.4615

>T120719 WP_050858704.1 NZ_CP038140:7363-7644 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMVAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSPWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T120719 NZ_CP038140:7363-7644 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGTCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTGCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCCGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10251.64 Da
• Isoelectric Point: 10.1734

>AT120719 WP_000079938.1 NZ_CP038140:7097-7366 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT120719 NZ_CP038140:7097-7366 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829FT18