Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4066542..4067118 Replicon chromosome
Accession NZ_CP038138
Organism Escherichia coli strain G3X16-2

Toxin (Protein)


Gene name shpA Uniprot ID A0A811CE22
Locus tag E3N82_RS19510 Protein ID WP_001402820.1
Coordinates 4066831..4067118 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag E3N82_RS19505 Protein ID WP_001402821.1
Coordinates 4066542..4066844 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3N82_RS19485 4062233..4062745 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
E3N82_RS19490 4063138..4065288 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
E3N82_RS19495 4065338..4065541 + 204 WP_000467859.1 YbdD/YjiX family protein -
E3N82_RS19500 4065552..4066508 + 957 WP_001295525.1 GTPase -
E3N82_RS19505 4066542..4066844 - 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
E3N82_RS19510 4066831..4067118 - 288 WP_001402820.1 BrnT family toxin Toxin
E3N82_RS19515 4067315..4068481 + 1167 WP_000800831.1 restriction endonuclease -
E3N82_RS19520 4068545..4070164 + 1620 WP_001029742.1 type I restriction-modification system subunit M -
E3N82_RS19525 4070154..4071560 + 1407 WP_001402819.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11169.62 Da        Isoelectric Point: 7.4697

>T120714 WP_001402820.1 NZ_CP038138:c4067118-4066831 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T120714 NZ_CP038138:c4067118-4066831 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGTGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT120714 WP_001402821.1 NZ_CP038138:c4066844-4066542 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT120714 NZ_CP038138:c4066844-4066542 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTATACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAGGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A811CE22

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References