Detailed information of TA system TA120713

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3618159..3618993
• Replicon: chromosome
• Accession: NZ_CP038138
• Organism: Escherichia coli strain G3X16-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2X7CAN1
• Locus tag: E3N82_RS17405
• Protein ID: WP_039025883.1
• Coordinates: 3618159..3618536 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2X5LBZ2
• Locus tag: E3N82_RS17410
• Protein ID: WP_039025882.1
• Coordinates: 3618625..3618993 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3N82_RS17375	3613417..3614373	+	957	WP_000121354.1	molybdenum cofactor insertion chaperone PaoD	-
E3N82_RS17380	3614424..3614762	+	339	Protein_3373	LysR family transcriptional regulator	-
E3N82_RS17385	3615100..3616419	+	1320	WP_000144688.1	site-specific integrase	-
E3N82_RS17390	3616512..3617360	-	849	WP_039025884.1	DUF4942 domain-containing protein	-
E3N82_RS17395	3617457..3617654	-	198	WP_000772024.1	DUF957 domain-containing protein	-
E3N82_RS17400	3617674..3618162	-	489	Protein_3377	hypothetical protein	-
E3N82_RS17405	3618159..3618536	-	378	WP_039025883.1	type IV toxin-antitoxin system toxin CbtA	Toxin
E3N82_RS17410	3618625..3618993	-	369	WP_039025882.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E3N82_RS17415	3619043..3619687	-	645	WP_039025881.1	hypothetical protein	-
E3N82_RS17420	3619702..3619923	-	222	WP_000692312.1	DUF987 domain-containing protein	-
E3N82_RS17425	3619992..3620468	-	477	WP_001186742.1	RadC family protein	-
E3N82_RS17430	3620483..3620968	-	486	WP_063073032.1	antirestriction protein	-
E3N82_RS17435	3621059..3621877	-	819	WP_001234702.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3588594..3629308	40714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14176.22 Da
• Isoelectric Point: 7.8523

>T120713 WP_039025883.1 NZ_CP038138:c3618536-3618159 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T120713 NZ_CP038138:c3618536-3618159 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCTGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGAGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13792.52 Da
• Isoelectric Point: 5.9582

>AT120713 WP_039025882.1 NZ_CP038138:c3618993-3618625 [Escherichia coli]
VSDTFHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT120713 NZ_CP038138:c3618993-3618625 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A2X7CAN1

Antitoxin

• AlphaFold DB: A0A2X5LBZ2