Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3427845..3428463 Replicon chromosome
Accession NZ_CP038138
Organism Escherichia coli strain G3X16-2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E3N82_RS16505 Protein ID WP_001291435.1
Coordinates 3428245..3428463 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E3N82_RS16500 Protein ID WP_000344800.1
Coordinates 3427845..3428219 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3N82_RS16490 3422934..3424127 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E3N82_RS16495 3424150..3427299 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
E3N82_RS16500 3427845..3428219 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E3N82_RS16505 3428245..3428463 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E3N82_RS16510 3428635..3429186 + 552 WP_000102564.1 maltose O-acetyltransferase -
E3N82_RS16515 3429302..3429772 + 471 WP_001402072.1 YlaC family protein -
E3N82_RS16520 3429936..3431486 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E3N82_RS16525 3431528..3431881 - 354 WP_000878140.1 DUF1428 domain-containing protein -
E3N82_RS16535 3432260..3432571 + 312 WP_000409911.1 MGMT family protein -
E3N82_RS16540 3432602..3433174 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T120712 WP_001291435.1 NZ_CP038138:3428245-3428463 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T120712 NZ_CP038138:3428245-3428463 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT120712 WP_000344800.1 NZ_CP038138:3427845-3428219 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT120712 NZ_CP038138:3427845-3428219 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References