Detailed information of TA system TA120700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1375147..1375772
• Replicon: chromosome
• Accession: NZ_CP038138
• Organism: Escherichia coli strain G3X16-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: E3N82_RS06675
• Protein ID: WP_000911330.1
• Coordinates: 1375374..1375772 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: E3N82_RS06670
• Protein ID: WP_000450524.1
• Coordinates: 1375147..1375374 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3N82_RS06645	1370950..1371420	-	471	WP_001402398.1	thioredoxin-dependent thiol peroxidase	-
E3N82_RS06650	1371420..1371992	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
E3N82_RS06655	1372138..1373016	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
E3N82_RS06660	1373033..1374067	+	1035	WP_001491244.1	outer membrane protein assembly factor BamC	-
E3N82_RS06665	1374280..1374993	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
E3N82_RS06670	1375147..1375374	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
E3N82_RS06675	1375374..1375772	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
E3N82_RS06680	1375919..1376782	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
E3N82_RS06685	1376797..1378812	+	2016	WP_136655682.1	tRNA cytosine(34) acetyltransferase TmcA	-
E3N82_RS06690	1378886..1379584	+	699	WP_000679812.1	esterase	-
E3N82_RS06695	1379694..1379894	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T120700 WP_000911330.1 NZ_CP038138:1375374-1375772 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T120700 NZ_CP038138:1375374-1375772 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT120700 WP_000450524.1 NZ_CP038138:1375147-1375374 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT120700 NZ_CP038138:1375147-1375374 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33