Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 897584..898238 Replicon chromosome
Accession NZ_CP038138
Organism Escherichia coli strain G3X16-2

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag E3N82_RS04405 Protein ID WP_000244777.1
Coordinates 897831..898238 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag E3N82_RS04400 Protein ID WP_000354046.1
Coordinates 897584..897850 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3N82_RS04375 892753..893496 + 744 WP_000951964.1 SDR family oxidoreductase -
E3N82_RS04380 893553..894986 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
E3N82_RS04385 895031..895342 + 312 WP_001402516.1 N(4)-acetylcytidine aminohydrolase -
E3N82_RS04390 895506..896165 + 660 WP_000250274.1 hemolysin III family protein -
E3N82_RS04395 896361..897341 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
E3N82_RS04400 897584..897850 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
E3N82_RS04405 897831..898238 + 408 WP_000244777.1 toxin CptA Toxin
E3N82_RS04410 898278..898799 - 522 WP_001402515.1 flavodoxin FldB -
E3N82_RS04415 898911..899807 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
E3N82_RS04420 899832..900542 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
E3N82_RS04425 900548..902281 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T120698 WP_000244777.1 NZ_CP038138:897831-898238 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T120698 NZ_CP038138:897831-898238 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT120698 WP_000354046.1 NZ_CP038138:897584-897850 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT120698 NZ_CP038138:897584-897850 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References