Detailed information of TA system TA120696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 9195..9742
• Replicon: plasmid pRAS3-3432
• Accession: NZ_CP038105
• Organism: Aeromonas salmonicida subsp. salmonicida strain SHY16-3432

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3D14_RS23980
• Protein ID: WP_009873354.1
• Coordinates: 9416..9742 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: E3D14_RS23975
• Protein ID: WP_043149934.1
• Coordinates: 9195..9419 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3D14_RS23955	4792..5475	-	684	WP_009873358.1	protein mobD	-
E3D14_RS23960	5488..5844	-	357	WP_009873357.1	hypothetical protein	-
E3D14_RS23965	6155..6466	+	312	WP_009873356.1	ribbon-helix-helix protein, CopG family	-
E3D14_RS23970	6456..9128	+	2673	WP_047353284.1	relaxase/mobilization nuclease domain-containing protein	-
E3D14_RS23975	9195..9419	+	225	WP_043149934.1	antitoxin MazE family protein	Antitoxin
E3D14_RS23980	9416..9742	+	327	WP_009873354.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3D14_RS23985	9739..10065	+	327	WP_009873353.1	hypothetical protein	-
E3D14_RS23990	10094..10972	+	879	WP_149604798.1	helicase RepA family protein	-
E3D14_RS23995	10953..11945	+	993	WP_009873351.1	plasmid replication	-
E3D14_RS24000	11998..13377	-	1380	WP_009873366.1	transposase	-
E3D14_RS24005	13454..13909	+	456	WP_009873365.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(C)	-	1..13912	13912

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11487.49 Da
• Isoelectric Point: 9.2954

>T120696 WP_009873354.1 NZ_CP038105:9416-9742 [Aeromonas salmonicida subsp. salmonicida]
MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTLNAAPLLRVTVQPSAENGLQKPSQVMVDKAMTVLRSK
VGPAFGRIDADALLEVERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T120696 NZ_CP038105:9416-9742 [Aeromonas salmonicida subsp. salmonicida]
ATGATGCGCGGCGACCTTGTGACCATCGCCATGCAGGGCGACTTTGGCAAGCCACGGCCTGCGCTTGTGGTTCAGGCCAA
CCAGTTCAGCGAGCACGGCAGCGTTACCGTGCTGCCTGTCACCAGCACACTTAATGCCGCGCCGTTGCTGCGCGTCACGG
TTCAGCCGAGCGCAGAGAACGGTTTGCAGAAGCCATCGCAGGTGATGGTCGACAAGGCGATGACGGTGCTGCGCAGCAAG
GTAGGGCCAGCTTTCGGGCGCATCGACGCGGACGCACTGCTTGAGGTTGAGCGTTGCTTGGCCGTGTTCTTGGGGATTGC
GAAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8468.56 Da
• Isoelectric Point: 5.0038

>AT120696 WP_043149934.1 NZ_CP038105:9195-9419 [Aeromonas salmonicida subsp. salmonicida]
MATAHVNQRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCLLVAQADSADTAMQQFMDEALADVDGWTE

Nucleotide

• Length: 225 bp

>AT120696 NZ_CP038105:9195-9419 [Aeromonas salmonicida subsp. salmonicida]
ATGGCAACAGCACACGTTAATCAGCGGGTTCAGAAGCATCGCGATGCATTACGCATGGCGGGGCTTCGTCCGGTTCAAAT
CTGGGTGCCGGATACGCGCCGCCCCGACTTCGCGGAGGAGTGCCGCCGCCAATGCCTGCTTGTGGCTCAGGCCGACAGCG
CCGACACCGCCATGCAGCAGTTCATGGATGAAGCCTTGGCAGACGTGGACGGCTGGACGGAATGA