Detailed information of TA system TA120690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 260137..261751
• Replicon: chromosome
• Accession: NZ_CP038034
• Organism: Achromobacter insolitus strain LCu2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: E2544_RS01220
• Protein ID: WP_149063486.1
• Coordinates: 260399..261751 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6S7FIA7
• Locus tag: E2544_RS01215
• Protein ID: WP_042795666.1
• Coordinates: 260137..260394 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2544_RS01200	255787..256818	+	1032	WP_042797454.1	sugar ABC transporter	-
E2544_RS01205	256811..258715	+	1905	WP_063639233.1	Vi polysaccharide transport protein VexE	-
E2544_RS01210	258728..259948	+	1221	WP_149063485.1	polysaccharide lyase family 1 protein	-
E2544_RS01215	260137..260394	+	258	WP_042795666.1	helix-turn-helix domain-containing protein	Antitoxin
E2544_RS01220	260399..261751	+	1353	WP_149063486.1	type II toxin-antitoxin system HipA family toxin	Toxin
E2544_RS01225	261782..262549	-	768	WP_149063487.1	helix-turn-helix transcriptional regulator	-
E2544_RS01230	262642..263763	+	1122	WP_042797453.1	MFS transporter	-
E2544_RS01235	263760..264431	-	672	WP_063639549.1	Crp/Fnr family transcriptional regulator	-
E2544_RS01240	264658..265872	+	1215	WP_088607648.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (11-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49377.53 Da
• Isoelectric Point: 7.2258

>T120690 WP_149063486.1 NZ_CP038034:260399-261751 [Achromobacter insolitus]
MGRRSHTQVLGLWMNGAYVGEWSNRPHAGEVLQYDKDWVASAPGRPLSLSLPFTPGNGPHRGDVVRAYFENLLPDSKEIR
ERVARRFRAGSTDAFALLAEIGRDCAGALQILPAGIAPADVASVQASPLNDAQVAQLLRSVLAPAPLGGLAIAEDDFRIS
IAGAQEKTALLRMDGQWCLPQGATPTTHILKLPLGLVGNMKFDLSESVENEWLCSEILAAYGMPVARAEVLSFEDMKVLA
VERFDRRWWDGEDGKRSLVRLPQEDMCQATGTPPHLKYEADGGPGIDRIMGLLETSRRSEHDRRTFFQAQVLFWMLCATD
GHAKNFSLFLRPGGAYELTPLYDVLSAYPILGEGPGKLSPVRAKMAMAVRSKNPHWRMRDIQRRHWLTVGARHGVVTPDG
QGVQFVVDDLIARTPEAVRAVRARLPQGFPQVLADSILDGLQAAADRLAA

Nucleotide

• Length: 1353 bp

>T120690 NZ_CP038034:260399-261751 [Achromobacter insolitus]
ATGGGGCGACGTTCACACACGCAGGTCCTGGGCCTATGGATGAATGGCGCCTATGTCGGAGAGTGGAGCAATCGACCCCA
CGCCGGCGAGGTCCTGCAGTACGACAAGGACTGGGTCGCCTCCGCGCCGGGCAGGCCATTGTCGCTGTCCCTGCCCTTCA
CGCCGGGCAATGGCCCGCATCGCGGCGACGTCGTCCGCGCATACTTCGAAAATCTGCTGCCTGACAGCAAGGAAATCCGC
GAACGGGTTGCGCGCCGGTTCCGGGCAGGCTCGACCGACGCGTTCGCCTTGCTTGCCGAGATCGGTCGCGATTGCGCGGG
CGCCTTGCAGATCCTGCCTGCGGGAATAGCGCCTGCAGACGTGGCGTCGGTGCAAGCCTCGCCCTTGAACGATGCCCAGG
TTGCGCAGCTGTTGCGCAGTGTTTTGGCGCCGGCGCCTCTCGGCGGCCTGGCCATCGCTGAGGATGATTTCCGGATTTCC
ATTGCAGGTGCGCAAGAGAAGACCGCCTTGCTGCGGATGGACGGCCAATGGTGCTTGCCCCAAGGCGCCACGCCGACGAC
TCACATCCTGAAGTTGCCATTGGGGCTGGTCGGCAATATGAAGTTCGACCTGAGCGAATCGGTGGAAAACGAATGGCTCT
GCTCGGAGATCTTGGCTGCCTACGGCATGCCCGTGGCGCGCGCGGAAGTACTGAGCTTCGAAGACATGAAGGTGCTCGCC
GTCGAACGTTTCGACCGCCGCTGGTGGGACGGCGAAGACGGAAAGCGCAGTCTAGTCCGCCTGCCCCAGGAAGACATGTG
CCAGGCCACCGGCACCCCGCCACACCTGAAGTACGAGGCGGACGGCGGGCCCGGCATCGACCGCATCATGGGTCTGCTCG
AAACTTCGCGTCGCTCAGAGCATGACCGGCGGACGTTCTTCCAGGCGCAGGTTCTGTTCTGGATGCTGTGCGCGACGGAC
GGCCACGCGAAGAACTTCAGCCTGTTCCTGCGGCCGGGCGGGGCCTACGAACTTACACCGCTATACGACGTGCTGTCCGC
CTATCCGATCCTGGGCGAGGGACCAGGCAAGCTGTCGCCCGTTCGAGCGAAGATGGCGATGGCCGTGCGTTCGAAGAACC
CGCATTGGCGCATGCGCGATATCCAGCGGCGCCACTGGTTGACGGTGGGCGCGCGACATGGCGTCGTGACGCCGGACGGC
CAGGGAGTCCAATTCGTCGTTGACGATTTGATCGCGCGTACGCCAGAGGCCGTGCGTGCGGTCCGCGCCAGATTGCCACA
AGGATTTCCGCAGGTGCTGGCGGACAGCATCCTGGACGGCTTGCAGGCCGCGGCGGACAGGCTCGCCGCGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9220.58 Da
• Isoelectric Point: 8.9814

>AT120690 WP_042795666.1 NZ_CP038034:260137-260394 [Achromobacter insolitus]
MDFTVRTATQLPALLQGFRKEAGLTQAATALRLGVTQQTLSALERNADKVSADRLLQLLSILGVEMVLRRQGAQPGPETT
SEPSW

Nucleotide

• Length: 258 bp

>AT120690 NZ_CP038034:260137-260394 [Achromobacter insolitus]
ATGGATTTCACCGTACGCACCGCGACACAATTGCCGGCCCTGCTCCAGGGGTTCCGCAAAGAGGCCGGCTTGACCCAGGC
GGCCACGGCCTTGCGCCTTGGGGTCACACAACAGACCTTGTCCGCCCTGGAGCGCAATGCCGACAAGGTCAGCGCAGACC
GGCTGCTGCAGCTGTTGAGCATCCTGGGAGTCGAAATGGTCTTGCGCCGGCAAGGGGCGCAGCCGGGACCCGAAACAACA
TCCGAACCCAGCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6S7FIA7