Detailed information of TA system TA120681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicA
• Location: 1020235..1020648
• Replicon: chromosome
• Accession: NZ_CP038033
• Organism: Nitrosococcus wardiae strain D1FHS

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: E3U44_RS04960
• Protein ID: WP_134359664.1
• Coordinates: 1020463..1020648 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E3U44_RS04955
• Protein ID: WP_134356941.1
• Coordinates: 1020235..1020450 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3U44_RS04935	1016870..1018453	-	1584	WP_134356938.1	DUF3459 domain-containing protein	-
E3U44_RS04940	1018781..1018972	-	192	WP_134356939.1	hypothetical protein	-
E3U44_RS04945	1019244..1019456	-	213	WP_134356940.1	cold-shock protein	-
E3U44_RS19190	1019688..1019909	-	222	WP_166804994.1	hypothetical protein	-
E3U44_RS04955	1020235..1020450	-	216	WP_134356941.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E3U44_RS04960	1020463..1020648	-	186	WP_134359664.1	type II toxin-antitoxin system HicA family toxin	Toxin
E3U44_RS04965	1020797..1020982	+	186	WP_206054893.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E3U44_RS04970	1020948..1021304	+	357	WP_206054894.1	HigA family addiction module antidote protein	-
E3U44_RS04975	1021597..1022739	-	1143	WP_134356943.1	transposase	-
E3U44_RS04980	1022793..1022930	-	138	WP_134356944.1	DUF4926 domain-containing protein	-
E3U44_RS04985	1023573..1024088	-	516	WP_134356945.1	DUF2141 domain-containing protein	-
E3U44_RS04990	1024533..1024697	-	165	WP_166804995.1	IS3 family transposase	-
E3U44_RS04995	1024764..1025024	-	261	WP_134359667.1	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-62)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6909.98 Da
• Isoelectric Point: 11.0174

>T120681 WP_134359664.1 NZ_CP038033:c1020648-1020463 [Nitrosococcus wardiae]
MKVRDILHKLKDDGWYLHSIRGSHRQFKHPSKLGRVTVSGKPGDDLAPGTINSIFKQAGWK

Nucleotide

• Length: 186 bp

>T120681 NZ_CP038033:c1020648-1020463 [Nitrosococcus wardiae]
ATGAAAGTCCGTGATATTCTCCATAAATTGAAAGACGACGGCTGGTACCTCCACTCAATTCGGGGTAGCCATCGCCAGTT
CAAACACCCGTCCAAGTTGGGCCGGGTCACAGTTTCGGGAAAGCCTGGTGATGATCTGGCACCCGGAACCATCAATAGCA
TTTTCAAGCAAGCTGGCTGGAAATAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7787.71 Da
• Isoelectric Point: 3.7930

>AT120681 WP_134356941.1 NZ_CP038033:c1020450-1020235 [Nitrosococcus wardiae]
MKYAIVIEKAASNYSAYVPDLPGCVATGSTIEEVESEIREAIEFHLEGMREDGELIPPPSSQVEYVDIDIA

Nucleotide

• Length: 216 bp

>AT120681 NZ_CP038033:c1020450-1020235 [Nitrosococcus wardiae]
ATGAAATATGCAATCGTTATTGAAAAGGCTGCAAGCAACTATTCTGCCTATGTCCCAGACCTCCCTGGGTGCGTAGCTAC
CGGTTCTACCATTGAGGAAGTCGAATCAGAGATTCGGGAAGCCATTGAGTTTCATCTTGAAGGAATGCGAGAGGATGGTG
AGCTAATTCCTCCACCAAGCAGTCAAGTTGAATACGTTGACATTGATATCGCCTAA