Detailed information of TA system TA120676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1346010..1346647
• Replicon: chromosome
• Accession: NZ_CP038028
• Organism: Bacillus amyloliquefaciens strain FS1092

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: E3U39_RS06725
• Protein ID: WP_003156187.1
• Coordinates: 1346010..1346360 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: E3U39_RS06730
• Protein ID: WP_003156188.1
• Coordinates: 1346366..1346647 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3U39_RS06685	1341050..1341652	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
E3U39_RS06690	1341652..1342440	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
E3U39_RS06695	1342406..1342888	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
E3U39_RS06700	1342885..1343214	-	330	WP_033575044.1	anti-sigma factor antagonist	-
E3U39_RS06705	1343278..1344285	-	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
E3U39_RS06710	1344297..1344698	-	402	WP_003156178.1	anti-sigma regulatory factor	-
E3U39_RS06715	1344701..1345066	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
E3U39_RS06720	1345071..1345892	-	822	WP_033575045.1	STAS domain-containing protein	-
E3U39_RS06725	1346010..1346360	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
E3U39_RS06730	1346366..1346647	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
E3U39_RS06735	1346767..1347936	-	1170	WP_053573676.1	alanine racemase	-
E3U39_RS06740	1348053..1349060	-	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
E3U39_RS06745	1349225..1349590	-	366	WP_015416872.1	holo-ACP synthase	-
E3U39_RS06750	1349683..1350282	+	600	WP_053573675.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T120676 WP_003156187.1 NZ_CP038028:c1346360-1346010 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T120676 NZ_CP038028:c1346360-1346010 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT120676 WP_003156188.1 NZ_CP038028:c1346647-1346366 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT120676 NZ_CP038028:c1346647-1346366 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5