Detailed information of TA system TA120673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 14825..15350
• Replicon: plasmid pAR3
• Accession: NZ_CP038025
• Organism: Acinetobacter radioresistens strain DD78

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R9B9L0
• Locus tag: E3H47_RS14985
• Protein ID: WP_005134734.1
• Coordinates: 14825..15112 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E3H47_RS14990
• Protein ID: WP_138001049.1
• Coordinates: 15102..15350 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3H47_RS14955	10120..10791	-	672	WP_005155746.1	hypothetical protein	-
E3H47_RS14960	10913..11113	-	201	WP_005155747.1	hypothetical protein	-
E3H47_RS14965	11162..12304	-	1143	WP_138001048.1	DUF3387 domain-containing protein	-
E3H47_RS14970	12268..12507	-	240	Protein_11	DNA methyltransferase	-
E3H47_RS14975	12762..13340	+	579	WP_004676046.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
E3H47_RS14980	13356..14660	+	1305	WP_004676048.1	Y-family DNA polymerase	-
E3H47_RS14985	14825..15112	-	288	WP_005134734.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3H47_RS14990	15102..15350	-	249	WP_138001049.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3H47_RS14995	15353..15814	-	462	WP_138001050.1	hypothetical protein	-
E3H47_RS15000	15884..16282	+	399	Protein_17	mechanosensitive ion channel family protein	-
E3H47_RS15005	16347..17648	-	1302	WP_016541981.1	MSMEG_0569 family flavin-dependent oxidoreductase	-
E3H47_RS15010	17659..17949	-	291	WP_138001051.1	MSMEG_0570 family nitrogen starvation response protein	-
E3H47_RS15015	17942..18988	-	1047	WP_138001075.1	sll0787 family AIR synthase-like protein	-
E3H47_RS15020	18988..19551	-	564	WP_002058091.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..80103	80103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11073.89 Da
• Isoelectric Point: 10.1961

>T120673 WP_005134734.1 NZ_CP038025:c15112-14825 [Acinetobacter radioresistens]
MTYKLDFLEEALAEWNKLNPSIKQPLKKKLIKVLENPRIPKNKLSGHPNRYKIKLRSIGYRLVYEVIDDEVVVIVIAVGR
RENNAVYDDANSRHS

Nucleotide

• Length: 288 bp

>T120673 NZ_CP038025:c15112-14825 [Acinetobacter radioresistens]
ATGACCTATAAGTTAGACTTTTTAGAAGAAGCATTAGCAGAATGGAATAAGCTGAATCCAAGTATTAAGCAGCCATTGAA
GAAAAAGTTGATCAAGGTTTTGGAGAATCCTCGTATTCCCAAGAATAAGCTGTCAGGGCATCCTAACCGCTACAAGATCA
AGCTTCGCAGCATTGGTTATCGTCTGGTTTATGAAGTCATTGATGATGAGGTAGTTGTCATCGTGATTGCTGTAGGCAGA
CGTGAAAACAATGCCGTCTATGATGATGCAAACTCTCGTCATTCATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9258.71 Da
• Isoelectric Point: 5.0491

>AT120673 WP_138001049.1 NZ_CP038025:c15350-15102 [Acinetobacter radioresistens]
MTQLIHARITASITELKKSPMDTVLAGKGEPVAILNRNTPAFYCVPADLYETMMEQLEDLELNKIADTRANQKRIRVNID
DL

Nucleotide

• Length: 249 bp

>AT120673 NZ_CP038025:c15350-15102 [Acinetobacter radioresistens]
ATGACTCAATTAATCCATGCACGAATAACTGCAAGTATTACTGAACTCAAAAAGAGTCCGATGGATACAGTGTTGGCAGG
AAAAGGGGAACCTGTCGCAATCCTCAATCGTAATACACCAGCGTTTTACTGTGTTCCGGCTGATCTGTATGAAACGATGA
TGGAACAACTAGAAGACCTGGAACTGAATAAGATTGCAGATACTCGTGCGAATCAGAAACGCATTCGAGTAAATATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: R9B9L0