Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2185172..2185701 Replicon chromosome
Accession NZ_CP038021
Organism Staphylococcus aureus strain 04-002

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag E3I93_RS11280 Protein ID WP_000621176.1
Coordinates 2185172..2185534 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag E3I93_RS11285 Protein ID WP_000948331.1
Coordinates 2185531..2185701 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3I93_RS14905 2182152..2182922 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
E3I93_RS14910 2182944..2183375 - 432 Protein_2090 anti-sigma B factor RsbW -
E3I93_RS11265 2183377..2183703 - 327 WP_001052491.1 anti-sigma factor antagonist -
E3I93_RS11270 2183822..2184823 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
E3I93_RS11280 2185172..2185534 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E3I93_RS11285 2185531..2185701 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
E3I93_RS11290 2185786..2186934 - 1149 WP_001281140.1 alanine racemase -
E3I93_RS11295 2187000..2187359 - 360 WP_000581197.1 holo-ACP synthase -
E3I93_RS11300 2187363..2187854 - 492 WP_001286801.1 PH domain-containing protein -
E3I93_RS11305 2187847..2189424 - 1578 WP_001294646.1 PH domain-containing protein -
E3I93_RS11310 2189417..2189896 - 480 WP_001287082.1 hypothetical protein -
E3I93_RS11315 2190105..2190665 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T120663 WP_000621176.1 NZ_CP038021:c2185534-2185172 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T120663 NZ_CP038021:c2185534-2185172 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT120663 WP_000948331.1 NZ_CP038021:c2185701-2185531 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT120663 NZ_CP038021:c2185701-2185531 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References