Detailed information of TA system TA120648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relE/YoeB-RelB
• Location: 1185755..1186257
• Replicon: chromosome
• Accession: NZ_CP038017
• Organism: Allofrancisella frigidaquae strain SYSU 10HL1970

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: E3E15_RS05555
• Protein ID: WP_172106906.1
• Coordinates: 1185755..1186009 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M3HUD0
• Locus tag: E3E15_RS05560
• Protein ID: WP_035719783.1
• Coordinates: 1186006..1186257 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3E15_RS05530	1181049..1181756	-	708	WP_035719772.1	ribonuclease PH	-
E3E15_RS05535	1181855..1182364	+	510	WP_172106902.1	chorismate lyase	-
E3E15_RS05540	1182361..1183215	+	855	WP_172106903.1	4-hydroxybenzoate octaprenyltransferase	-
E3E15_RS05545	1183461..1184003	-	543	WP_172106904.1	hypothetical protein	-
E3E15_RS05550	1184005..1185168	-	1164	WP_172106905.1	hypothetical protein	-
E3E15_RS05555	1185755..1186009	-	255	WP_172106906.1	Txe/YoeB family addiction module toxin	Toxin
E3E15_RS05560	1186006..1186257	-	252	WP_035719783.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
E3E15_RS05565	1186428..1187168	-	741	WP_172106907.1	Fic family protein	-
E3E15_RS05570	1187257..1187463	-	207	WP_172106908.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
E3E15_RS05575	1187426..1187833	-	408	WP_172106909.1	hypothetical protein	-
E3E15_RS05580	1187951..1188544	-	594	WP_172106910.1	recombinase family protein	-
E3E15_RS05585	1188569..1188799	-	231	WP_172107240.1	DUF2442 domain-containing protein	-
E3E15_RS05590	1188833..1189090	-	258	WP_172106911.1	DUF4160 domain-containing protein	-
E3E15_RS05595	1189162..1189314	-	153	WP_155960878.1	Fic family protein	-
E3E15_RS05600	1189577..1189882	+	306	WP_209451674.1	helix-turn-helix domain-containing protein	-
E3E15_RS05605	1189879..1191078	+	1200	WP_172106912.1	type II toxin-antitoxin system HipA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10338.88 Da
• Isoelectric Point: 8.7732

>T120648 WP_172106906.1 NZ_CP038017:c1186009-1185755 [Allofrancisella frigidaquae]
MKLLFTDEAWEEYLYWQSYDKKIIKKINSLIKEVKKSPFEGTGNPEPLKYDLSGFWSRRIDREHRLVYKIDNDTLFIVQC
RYHY

Nucleotide

• Length: 255 bp

>T120648 NZ_CP038017:c1186009-1185755 [Allofrancisella frigidaquae]
ATGAAACTCTTATTTACAGATGAAGCATGGGAAGAGTATTTATATTGGCAAAGCTATGACAAAAAAATAATAAAAAAAAT
AAATAGTTTAATTAAGGAAGTAAAAAAAAGCCCTTTTGAAGGCACGGGCAATCCTGAACCATTAAAATATGATCTATCAG
GATTTTGGTCAAGAAGAATAGATAGAGAACATAGGTTAGTCTATAAAATCGATAATGATACTCTTTTTATTGTTCAGTGT
AGATACCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9676.95 Da
• Isoelectric Point: 5.8718

>AT120648 WP_035719783.1 NZ_CP038017:c1186257-1186006 [Allofrancisella frigidaquae]
MKTATYSFFREKLSSMLDFVNENHEPLIITRKNGSNAVLISLDDFNSYEETSYLMRSPKNAERLRKSISSLEDGKIKERE
LLE

Nucleotide

• Length: 252 bp

>AT120648 NZ_CP038017:c1186257-1186006 [Allofrancisella frigidaquae]
ATGAAAACAGCTACATATTCTTTCTTTAGAGAAAAATTATCCAGTATGCTAGATTTTGTTAATGAAAATCATGAGCCACT
TATAATAACCAGAAAAAATGGTTCAAATGCAGTTTTAATTTCTTTAGATGATTTTAACTCATACGAAGAAACTTCGTACT
TAATGCGTAGTCCTAAGAATGCTGAGAGATTAAGAAAATCTATAAGCTCTTTAGAAGATGGTAAAATCAAAGAACGTGAG
CTGTTAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6M3HUD0