Detailed information of TA system TA120645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 428387..429023
• Replicon: chromosome
• Accession: NZ_CP038015
• Organism: Paenisporosarcina antarctica strain CGMCC 1.6503

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: S2YDG5
• Locus tag: E2636_RS02195
• Protein ID: WP_016429759.1
• Coordinates: 428673..429023 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: E2636_RS02190
• Protein ID: WP_026045638.1
• Coordinates: 428387..428668 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2636_RS02165	423460..424983	+	1524	WP_208324130.1	PH domain-containing protein	-
E2636_RS02170	424995..425612	-	618	WP_134208610.1	rhomboid family intramembrane serine protease	-
E2636_RS02175	425683..426036	+	354	WP_134208612.1	holo-ACP synthase	-
E2636_RS02180	426095..427102	+	1008	WP_134208614.1	outer membrane lipoprotein carrier protein LolA	-
E2636_RS02185	427147..428256	+	1110	WP_134208617.1	alanine racemase	-
E2636_RS02190	428387..428668	+	282	WP_026045638.1	hypothetical protein	Antitoxin
E2636_RS02195	428673..429023	+	351	WP_016429759.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E2636_RS02200	429167..429991	+	825	WP_017380016.1	STAS domain-containing protein	-
E2636_RS02205	429994..430356	+	363	WP_134208619.1	STAS domain-containing protein	-
E2636_RS02210	430359..430760	+	402	WP_134208621.1	anti-sigma regulatory factor	-
E2636_RS02215	430775..431779	+	1005	WP_134208623.1	PP2C family protein-serine/threonine phosphatase	-
E2636_RS02220	431854..432183	+	330	WP_134208626.1	anti-sigma factor antagonist	-
E2636_RS02225	432186..432656	+	471	WP_134208628.1	anti-sigma B factor RsbW	-
E2636_RS02230	432634..433422	+	789	WP_134208631.1	RNA polymerase sigma factor SigB	-
E2636_RS02235	433419..434018	+	600	WP_134208632.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12880.88 Da
• Isoelectric Point: 4.8838

>T120645 WP_016429759.1 NZ_CP038015:428673-429023 [Paenisporosarcina antarctica]
MNVKRGDVFFADLSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQL
RTIDKSRLTDKITQLDDVLMEEVDEALEISLGLVKF

Nucleotide

• Length: 351 bp

>T120645 NZ_CP038015:428673-429023 [Paenisporosarcina antarctica]
TTGAACGTAAAACGTGGTGACGTTTTTTTTGCTGACTTATCGCCAGTAGTCGGGTCTGAACAAGGTGGGACTAGACCCGT
TCTGGTGATTCAAAACGATATCGGAAATCGTTTCAGCCCAACAGTCATCATTGCGGCTATCACGGCGCAAATTCAAAAAG
CAAAATTACCAACACATGTGGAAATCAATGCTAAAAAGTATGGATTTGAACGGGATTCCGTTATTTTGCTTGAACAATTG
CGTACGATTGATAAATCGCGGCTGACTGATAAAATTACTCAGCTGGATGATGTTCTGATGGAAGAAGTCGATGAGGCGCT
AGAAATAAGTCTTGGTCTTGTAAAGTTTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10770.47 Da
• Isoelectric Point: 8.9120

>AT120645 WP_026045638.1 NZ_CP038015:428387-428668 [Paenisporosarcina antarctica]
VNEKRKTKEVIVKLPRQFVNNLDKQKEQQLAKPEEFVYISTKKSIKQVQPNLIREAMIKGYVEMSQINLSIASECLHLEY
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT120645 NZ_CP038015:428387-428668 [Paenisporosarcina antarctica]
GTGAATGAAAAGAGAAAAACAAAGGAAGTTATTGTGAAACTTCCAAGACAATTTGTGAACAATCTTGATAAACAGAAAGA
ACAACAACTGGCTAAGCCAGAAGAGTTTGTTTATATTTCGACAAAAAAATCAATTAAACAAGTTCAACCAAATCTTATAC
GAGAAGCGATGATCAAGGGCTACGTAGAAATGTCGCAGATTAACTTGTCCATTGCAAGCGAGTGTCTGCATTTGGAATAT
GAAGCGGAGCATACTGTGGAGCGACTTGTAAGCGGAGGATGA

Structures

Toxin

• AlphaFold DB: S2YDG5