Detailed information of TA system TA120644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 3135378..3135996
• Replicon: chromosome
• Accession: NZ_CP038012
• Organism: Sporosarcina pasteurii strain BNCC 337394

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A380CGL8
• Locus tag: E2C16_RS15140
• Protein ID: WP_075526783.1
• Coordinates: 3135646..3135996 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: E2C16_RS15135
• Protein ID: WP_115364178.1
• Coordinates: 3135378..3135641 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2C16_RS15115	3131810..3132421	-	612	WP_115363356.1	rhomboid family intramembrane serine protease	-
E2C16_RS15120	3132488..3132841	+	354	WP_115363354.1	holo-ACP synthase	-
E2C16_RS15125	3132900..3133997	+	1098	WP_115363352.1	outer membrane lipoprotein carrier protein LolA	-
E2C16_RS15130	3134088..3135215	+	1128	WP_115363350.1	alanine racemase	-
E2C16_RS15135	3135378..3135641	+	264	WP_115364178.1	transcriptional regulator	Antitoxin
E2C16_RS15140	3135646..3135996	+	351	WP_075526783.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E2C16_RS15145	3136145..3136975	+	831	WP_115363348.1	STAS domain-containing protein	-
E2C16_RS15150	3136972..3137334	+	363	WP_115363346.1	STAS domain-containing protein	-
E2C16_RS15155	3137337..3137738	+	402	WP_115363344.1	anti-sigma regulatory factor	-
E2C16_RS15160	3137754..3138761	+	1008	WP_115363342.1	PP2C family protein-serine/threonine phosphatase	-
E2C16_RS15165	3138922..3139263	+	342	WP_115364177.1	STAS domain-containing protein	-
E2C16_RS15170	3139250..3139717	+	468	WP_115363340.1	anti-sigma B factor RsbW	-
E2C16_RS15175	3139695..3140483	+	789	WP_115363337.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12985.01 Da
• Isoelectric Point: 4.9105

>T120644 WP_075526783.1 NZ_CP038012:3135646-3135996 [Sporosarcina pasteurii]
MAIKRGDVFFADLSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAEKYQFERDSVILLEQV
RTIDKSRLTDKITHLDEMLMEKVDEALEVSFGLVKF

Nucleotide

• Length: 351 bp

>T120644 NZ_CP038012:3135646-3135996 [Sporosarcina pasteurii]
TTGGCAATAAAACGAGGAGACGTCTTTTTTGCAGACCTGTCGCCGGTCGTTGGTTCTGAGCAAGGGGGTACCAGACCGGT
CCTTGTCATACAAAATGACATTGGGAACCGGTTTAGTCCAACTGTAATTATCGCAGCCATTACTGCACAGATTCAGAAAG
CAAAGTTGCCGACACATGTAGAAATCGATGCGGAAAAGTATCAATTCGAGCGGGACTCTGTAATCCTGCTCGAACAAGTC
CGCACGATTGATAAGTCGAGGTTAACTGATAAAATTACGCATCTTGATGAAATGTTGATGGAAAAAGTCGACGAAGCATT
AGAAGTAAGTTTTGGGCTCGTAAAATTTTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.58 Da
• Isoelectric Point: 6.3281

>AT120644 WP_115364178.1 NZ_CP038012:3135378-3135641 [Sporosarcina pasteurii]
MREVIVKIPKRMLNENEHTVVHQGQERGDFVYISTKRYETNHEPEAIREAMMKGYVEMSQINLKIATECLHVEFEAQHTV
ERLVSGG

Nucleotide

• Length: 264 bp

>AT120644 NZ_CP038012:3135378-3135641 [Sporosarcina pasteurii]
ATGAGAGAGGTTATTGTTAAAATTCCGAAACGAATGTTGAATGAAAATGAACATACGGTCGTCCACCAAGGGCAGGAGCG
AGGGGATTTTGTATATATTTCGACAAAACGCTATGAGACCAATCATGAACCTGAGGCAATTCGAGAGGCCATGATGAAAG
GGTATGTTGAAATGTCGCAAATTAACTTAAAAATTGCCACGGAATGTTTACATGTGGAATTTGAAGCTCAGCACACAGTG
GAACGTCTCGTTAGCGGAGGATGA

Structures

Toxin

• AlphaFold DB: A0A380CGL8