Detailed information of TA system TA120642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 269343..269927
• Replicon: plasmid pAHTJR1
• Accession: NZ_CP038010
• Organism: Acinetobacter haemolyticus strain TJR01

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: AHTJR_RS17455
• Protein ID: WP_000286964.1
• Coordinates: 269343..269645 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AHTJR_RS17460
• Protein ID: WP_000985609.1
• Coordinates: 269646..269927 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AHTJR_RS17430	265102..266267	+	1166	WP_085944217.1	IS3-like element ISAba22 family transposase	-
AHTJR_RS17435	266405..267730	+	1326	WP_134253135.1	ISNCY family transposase	-
AHTJR_RS17440	267987..268274	+	288	WP_000438825.1	BrnT family toxin	-
AHTJR_RS17445	268261..268575	+	315	WP_004728120.1	BrnA antitoxin family protein	-
AHTJR_RS17450	268664..269194	-	531	WP_000172759.1	hypothetical protein	-
AHTJR_RS17455	269343..269645	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AHTJR_RS17460	269646..269927	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
AHTJR_RS17465	270007..270426	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
AHTJR_RS17470	270567..270887	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AHTJR_RS17475	270880..271151	+	272	Protein_270	helix-turn-helix domain-containing protein	-
AHTJR_RS17480	271643..273117	+	1475	Protein_271	ABC-F type ribosomal protection protein Msr(E)	-
AHTJR_RS17485	273173..274056	+	884	Protein_272	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / tet(Y) / aph(3')-Ia / sul2 / ARR-3 / qacE / sul1 / blaPER-1 / aph(3')-VIa / blaOXA-58 / msr(E) / mph(E) / floR	-	1..306131	306131
	-	inside	IScluster/Tn	msr(E) / mph(E) / floR	-	265102..279313	14211

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T120642 WP_000286964.1 NZ_CP038010:269343-269645 [Acinetobacter haemolyticus]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T120642 NZ_CP038010:269343-269645 [Acinetobacter haemolyticus]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT120642 WP_000985609.1 NZ_CP038010:269646-269927 [Acinetobacter haemolyticus]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT120642 NZ_CP038010:269646-269927 [Acinetobacter haemolyticus]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94