Detailed information of TA system TA120641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 267987..268575
• Replicon: plasmid pAHTJR1
• Accession: NZ_CP038010
• Organism: Acinetobacter haemolyticus strain TJR01

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: AHTJR_RS17440
• Protein ID: WP_000438825.1
• Coordinates: 267987..268274 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: AHTJR_RS17445
• Protein ID: WP_004728120.1
• Coordinates: 268261..268575 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AHTJR_RS17420	263489..264085	+	597	WP_074163998.1	LysE family transporter	-
AHTJR_RS17425	264113..264930	-	818	Protein_260	AraC family transcriptional regulator	-
AHTJR_RS17430	265102..266267	+	1166	WP_085944217.1	IS3-like element ISAba22 family transposase	-
AHTJR_RS17435	266405..267730	+	1326	WP_134253135.1	ISNCY family transposase	-
AHTJR_RS17440	267987..268274	+	288	WP_000438825.1	BrnT family toxin	Toxin
AHTJR_RS17445	268261..268575	+	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
AHTJR_RS17450	268664..269194	-	531	WP_000172759.1	hypothetical protein	-
AHTJR_RS17455	269343..269645	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AHTJR_RS17460	269646..269927	+	282	WP_000985609.1	putative addiction module antidote protein	-
AHTJR_RS17465	270007..270426	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
AHTJR_RS17470	270567..270887	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AHTJR_RS17475	270880..271151	+	272	Protein_270	helix-turn-helix domain-containing protein	-
AHTJR_RS17480	271643..273117	+	1475	Protein_271	ABC-F type ribosomal protection protein Msr(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / tet(Y) / aph(3')-Ia / sul2 / ARR-3 / qacE / sul1 / blaPER-1 / aph(3')-VIa / blaOXA-58 / msr(E) / mph(E) / floR	-	1..306131	306131
	-	inside	IScluster/Tn	msr(E) / mph(E) / floR	-	265102..279313	14211

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T120641 WP_000438825.1 NZ_CP038010:267987-268274 [Acinetobacter haemolyticus]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T120641 NZ_CP038010:267987-268274 [Acinetobacter haemolyticus]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT120641 WP_004728120.1 NZ_CP038010:268261-268575 [Acinetobacter haemolyticus]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT120641 NZ_CP038010:268261-268575 [Acinetobacter haemolyticus]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9