Detailed information of TA system TA120637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 6554558..6555197
• Replicon: chromosome
• Accession: NZ_CP038001
• Organism: Pseudomonas sp. SXM-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: DCC84_RS31005
• Protein ID: WP_134327413.1
• Coordinates: 6555015..6555197 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2N7Y8H9
• Locus tag: DCC84_RS31000
• Protein ID: WP_003211020.1
• Coordinates: 6554558..6554959 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCC84_RS30985	6549975..6553718	+	3744	WP_134327411.1	bifunctional diguanylate cyclase/phosphodiesterase	-
DCC84_RS30995	6553956..6554468	+	513	WP_134327412.1	cupin domain-containing protein	-
DCC84_RS31000	6554558..6554959	-	402	WP_003211020.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DCC84_RS31005	6555015..6555197	-	183	WP_134327413.1	type II toxin-antitoxin system HicA family toxin	Toxin
DCC84_RS31010	6555383..6556636	-	1254	WP_134327414.1	amidase	-
DCC84_RS31015	6556647..6557072	-	426	WP_003211024.1	VOC family protein	-
DCC84_RS31020	6557186..6558052	+	867	WP_134327415.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6587.68 Da
• Isoelectric Point: 10.9062

>T120637 WP_134327413.1 NZ_CP038001:c6555197-6555015 [Pseudomonas sp. SXM-1]
MRSREVIDLLMADGWFEVAAKGSHHQFKHSTKPGRVTVPHPKSEIAKGTLHGILKSAGLK

Nucleotide

• Length: 183 bp

>T120637 NZ_CP038001:c6555197-6555015 [Pseudomonas sp. SXM-1]
ATGAGAAGCAGAGAGGTAATTGACTTGCTGATGGCGGATGGATGGTTTGAGGTGGCGGCGAAAGGCAGTCACCATCAATT
CAAGCACTCAACCAAGCCCGGCCGGGTGACGGTTCCTCATCCTAAATCAGAGATAGCCAAGGGGACCTTGCACGGCATTT
TGAAATCAGCAGGCCTGAAGTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14726.54 Da
• Isoelectric Point: 4.5353

>AT120637 WP_003211020.1 NZ_CP038001:c6554959-6554558 [Pseudomonas sp. SXM-1]
MKFPVVIHKDADSDYSVTVPDVPGCFSAGTTFSEALDNVREALALHLEGLVADEERLPQAREVDVHLENPDFAGGVWAVI
DFDLTPYLGKSVRFNASLPEQLLNRIDERVQKDHRYASRSGFLATAALRELSV

Nucleotide

• Length: 402 bp

>AT120637 NZ_CP038001:c6554959-6554558 [Pseudomonas sp. SXM-1]
ATGAAATTTCCAGTGGTGATACACAAAGACGCTGATTCAGATTACAGCGTGACCGTCCCGGACGTGCCAGGTTGCTTTTC
TGCGGGCACAACCTTTTCCGAAGCTCTGGATAATGTCCGCGAAGCTTTAGCGCTGCACCTCGAGGGATTGGTTGCTGATG
AGGAGCGGCTTCCTCAGGCTAGAGAGGTCGACGTCCATTTGGAGAATCCGGACTTTGCGGGTGGCGTGTGGGCGGTGATC
GACTTTGATCTCACTCCTTACCTGGGCAAGTCAGTCAGGTTCAACGCCTCACTGCCTGAGCAACTTTTGAATCGTATCGA
TGAGCGAGTGCAAAAAGACCATCGCTATGCCTCGCGCTCTGGGTTCCTGGCGACGGCGGCTTTGCGCGAACTTTCCGTTT
GA

Structures

Antitoxin

• AlphaFold DB: A0A2N7Y8H9