Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4341426..4342080 Replicon chromosome
Accession NZ_CP037996
Organism Shigella flexneri strain FC906

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag CBL25_RS22260 Protein ID WP_000244767.1
Coordinates 4341426..4341833 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag CBL25_RS22265 Protein ID WP_000354052.1
Coordinates 4341814..4342080 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CBL25_RS22240 (4337383) 4337383..4339116 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
CBL25_RS22245 (4339122) 4339122..4339832 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CBL25_RS22250 (4339857) 4339857..4340753 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
CBL25_RS22255 (4340865) 4340865..4341386 + 522 WP_001055867.1 flavodoxin FldB -
CBL25_RS22260 (4341426) 4341426..4341833 - 408 WP_000244767.1 protein YgfX Toxin
CBL25_RS22265 (4341814) 4341814..4342080 - 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
CBL25_RS22270 (4342323) 4342323..4343303 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CBL25_RS22275 (4343499) 4343499..4344158 - 660 WP_000250274.1 hemolysin III family protein -
CBL25_RS22280 (4344322) 4344322..4344633 - 312 WP_001182950.1 N(4)-acetylcytidine aminohydrolase -
CBL25_RS22285 (4344678) 4344678..4346111 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
CBL25_RS22290 (4346168) 4346168..4346910 - 743 Protein_4257 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T120626 WP_000244767.1 NZ_CP037996:c4341833-4341426 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T120626 NZ_CP037996:c4341833-4341426 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT120626 WP_000354052.1 NZ_CP037996:c4342080-4341814 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT120626 NZ_CP037996:c4342080-4341814 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGTCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References