Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 2436533..2437328 | Replicon | chromosome |
| Accession | NZ_CP037996 | ||
| Organism | Shigella flexneri strain FC906 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | Q93EZ7 |
| Locus tag | CBL25_RS12175 | Protein ID | WP_000854917.1 |
| Coordinates | 2436954..2437328 (+) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | D2ACA1 |
| Locus tag | CBL25_RS12170 | Protein ID | WP_001280958.1 |
| Coordinates | 2436533..2436907 (+) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CBL25_RS12125 (2431946) | 2431946..2432362 | + | 417 | WP_000275180.1 | AraC family transcriptional regulator | - |
| CBL25_RS12130 (2432421) | 2432421..2433118 | + | 698 | WP_095033700.1 | IS1-like element IS1B family transposase | - |
| CBL25_RS24450 (2433301) | 2433301..2433564 | + | 264 | WP_000466628.1 | DNA polymerase III subunit gamma/tau | - |
| CBL25_RS12140 (2433684) | 2433684..2433857 | + | 174 | WP_000088744.1 | DUF905 family protein | - |
| CBL25_RS12145 (2433958) | 2433958..2434779 | + | 822 | WP_001234584.1 | DUF932 domain-containing protein | - |
| CBL25_RS12155 (2435121) | 2435121..2435594 | + | 474 | WP_005011597.1 | antirestriction protein | - |
| CBL25_RS12160 (2435610) | 2435610..2436086 | + | 477 | WP_005011624.1 | RadC family protein | - |
| CBL25_RS12165 (2436149) | 2436149..2436370 | + | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| CBL25_RS12170 (2436533) | 2436533..2436907 | + | 375 | WP_001280958.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| CBL25_RS12175 (2436954) | 2436954..2437328 | + | 375 | WP_000854917.1 | TA system toxin CbtA family protein | Toxin |
| CBL25_RS12180 (2437325) | 2437325..2437816 | + | 492 | WP_000976828.1 | DUF5983 family protein | - |
| CBL25_RS12185 (2437828) | 2437828..2438025 | + | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| CBL25_RS12190 (2438110) | 2438110..2438670 | + | 561 | Protein_2330 | DUF4942 domain-containing protein | - |
| CBL25_RS12195 (2438742) | 2438742..2439746 | + | 1005 | WP_031597378.1 | IS110-like element ISSfl8 family transposase | - |
| CBL25_RS12200 (2440030) | 2440030..2440311 | + | 282 | Protein_2332 | DUF4942 domain-containing protein | - |
| CBL25_RS12210 (2440782) | 2440782..2441720 | + | 939 | Protein_2333 | glyoxylate/hydroxypyruvate reductase GhrA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | catA1 / tet(B) | - | 2422597..2441255 | 18658 | |
| - | flank | IS/Tn | - | - | 2438742..2439746 | 1004 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14145.22 Da Isoelectric Point: 7.7761
>T120611 WP_000854917.1 NZ_CP037996:2436954-2437328 [Shigella flexneri]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T120611 NZ_CP037996:2436954-2437328 [Shigella flexneri]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13793.62 Da Isoelectric Point: 6.4651
>AT120611 WP_001280958.1 NZ_CP037996:2436533-2436907 [Shigella flexneri]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT120611 NZ_CP037996:2436533-2436907 [Shigella flexneri]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q93EZ7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0K4PAV2 |