Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 299419..300050 Replicon chromosome
Accession NZ_CP037996
Organism Shigella flexneri strain FC906

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag CBL25_RS01590 Protein ID WP_001259384.1
Coordinates 299775..300050 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CBL25_RS01585 Protein ID WP_000593555.1
Coordinates 299419..299778 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CBL25_RS01560 (295551) 295551..296495 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
CBL25_RS01565 (296492) 296492..297325 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
CBL25_RS01570 (297325) 297325..298089 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
CBL25_RS01575 (298086) 298086..298892 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
CBL25_RS01580 (298898) 298898..299299 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CBL25_RS01585 (299419) 299419..299778 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CBL25_RS01590 (299775) 299775..300050 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
CBL25_RS01595 (300123) 300123..301247 - 1125 WP_001216257.1 ABC transporter permease -
CBL25_RS01600 (301247) 301247..303982 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
CBL25_RS01605 (303979) 303979..305046 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T120605 WP_001259384.1 NZ_CP037996:c300050-299775 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T120605 NZ_CP037996:c300050-299775 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT120605 WP_000593555.1 NZ_CP037996:c299778-299419 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT120605 NZ_CP037996:c299778-299419 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References