Detailed information of TA system TA120602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 3175155..3175851
• Replicon: chromosome
• Accession: NZ_CP037993
• Organism: Herbaspirillum huttiense strain NFYY 53159

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: E2K99_RS14335
• Protein ID: WP_121041617.1
• Coordinates: 3175155..3175535 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: E2K99_RS14340
• Protein ID: WP_121041619.1
• Coordinates: 3175516..3175851 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2K99_RS14305	3170169..3171191	-	1023	WP_134222655.1	TRAP transporter substrate-binding protein DctP	-
E2K99_RS14310	3171339..3172007	+	669	WP_134222656.1	GntR family transcriptional regulator	-
E2K99_RS14315	3172026..3172925	-	900	WP_134222657.1	pseudouridine synthase	-
E2K99_RS14320	3172922..3174259	-	1338	WP_134222658.1	type II toxin-antitoxin system HipA family toxin	-
E2K99_RS14325	3174256..3174558	-	303	WP_039785683.1	helix-turn-helix domain-containing protein	-
E2K99_RS14330	3174759..3174947	-	189	WP_039785682.1	YjfB family protein	-
E2K99_RS14335	3175155..3175535	+	381	WP_121041617.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E2K99_RS14340	3175516..3175851	+	336	WP_121041619.1	helix-turn-helix domain-containing protein	Antitoxin
E2K99_RS14345	3175934..3176122	-	189	WP_034331488.1	hypothetical protein	-
E2K99_RS14350	3176333..3177184	-	852	WP_134222659.1	anti-sigma factor	-
E2K99_RS14355	3177181..3177738	-	558	WP_051156335.1	RNA polymerase sigma factor	-
E2K99_RS14360	3177765..3178136	-	372	WP_134222660.1	hypothetical protein	-
E2K99_RS14365	3178239..3178685	-	447	WP_134222661.1	group 1 truncated hemoglobin	-
E2K99_RS14370	3178702..3179727	-	1026	WP_134222662.1	DUF3034 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-97)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14408.72 Da
• Isoelectric Point: 9.8092

>T120602 WP_121041617.1 NZ_CP037993:3175155-3175535 [Herbaspirillum huttiense]
MFINEKPLEWIASSYKDLLALPAEVRQRFGFALSLAQMGERYEKTKILKGFSGAGVLEILEDHIGDTFRAVYTVKFEEAV
FVLHCFQKKSKSGIATPQPDMEIIRTRLKIATARAEELRHHAKNNR

Nucleotide

• Length: 381 bp

>T120602 NZ_CP037993:3175155-3175535 [Herbaspirillum huttiense]
ATGTTTATCAATGAGAAACCACTCGAATGGATCGCAAGCAGCTACAAGGATCTGCTGGCATTGCCAGCGGAAGTCCGCCA
GCGTTTCGGCTTCGCATTGTCGCTGGCGCAAATGGGCGAGCGCTATGAAAAAACCAAGATATTGAAGGGTTTCAGCGGTG
CTGGCGTGCTGGAGATTCTGGAAGACCATATTGGCGACACCTTCCGGGCTGTCTACACCGTGAAGTTCGAAGAAGCCGTG
TTCGTACTGCACTGCTTTCAGAAGAAAAGCAAAAGCGGAATAGCCACACCCCAGCCGGACATGGAAATCATCCGAACCAG
ACTCAAGATCGCTACTGCCAGAGCCGAGGAGCTACGTCATCATGCAAAAAACAATCGTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12004.12 Da
• Isoelectric Point: 9.9001

>AT120602 WP_121041619.1 NZ_CP037993:3175516-3175851 [Herbaspirillum huttiense]
MQKTIVEGTDILRSSGNVFADLGLPDADKLRIKTGLVIEIRKAMRALGLSQQAAAQRMGIPQPKVSGMMRGDFSNLSERK
LMDCLTRLGYDIEIQVRPAAASLGCLTLTVA

Nucleotide

• Length: 336 bp

>AT120602 NZ_CP037993:3175516-3175851 [Herbaspirillum huttiense]
ATGCAAAAAACAATCGTTGAGGGAACCGACATCCTCCGCAGCAGCGGCAATGTCTTCGCCGATCTTGGTCTGCCGGATGC
CGACAAGCTGCGCATCAAGACTGGTCTGGTCATCGAAATCCGCAAGGCGATGCGTGCACTGGGACTGAGTCAACAGGCGG
CGGCGCAACGCATGGGGATTCCACAACCAAAAGTGTCGGGCATGATGCGCGGCGACTTCAGCAACCTGTCCGAGCGCAAG
CTGATGGACTGTCTGACACGGCTGGGCTACGACATCGAGATCCAGGTGCGGCCTGCCGCCGCCTCCCTCGGATGCCTGAC
CTTGACCGTAGCCTGA