Detailed information of TA system TA120592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2088011..2089764
• Replicon: chromosome
• Accession: NZ_CP037971
• Organism: Burkholderia pseudomallei strain BPs133 isolate Melioidosis patient

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q63JY0
• Locus tag: E2R25_RS10540
• Protein ID: WP_004188815.1
• Coordinates: 2088412..2089764 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: E2R25_RS10535
• Protein ID: WP_131116773.1
• Coordinates: 2088011..2088415 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2R25_RS10520	2083023..2084543	-	1521	WP_134162199.1	cellulase	-
E2R25_RS10525	2084548..2086944	-	2397	WP_134162200.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
E2R25_RS36485	2087410..2087673	+	264	WP_004199789.1	hypothetical protein	-
E2R25_RS10535	2088011..2088415	+	405	WP_131116773.1	helix-turn-helix domain-containing protein	Antitoxin
E2R25_RS10540	2088412..2089764	+	1353	WP_004188815.1	type II toxin-antitoxin system HipA family toxin	Toxin
E2R25_RS10545	2089932..2090384	-	453	WP_004198842.1	hypothetical protein	-
E2R25_RS36490	2090485..2090631	-	147	WP_004199790.1	hypothetical protein	-
E2R25_RS10555	2090794..2091708	-	915	WP_004545706.1	LysR family transcriptional regulator	-
E2R25_RS10560	2091875..2092828	+	954	WP_004187785.1	agmatinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-408)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49848.83 Da
• Isoelectric Point: 9.6807

>T120592 WP_004188815.1 NZ_CP037971:2088412-2089764 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTADVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T120592 NZ_CP037971:2088412-2089764 [Burkholderia pseudomallei]
GTGAGCGCCCGCCGCGCACGCGCGACGCGCCTGCACCTGTGGATGAACGGCCTGCCCGTCGGCTACTGGGAGCACGCGCG
CGACGGCGAGCGCCTTGTCTACTTCGACGAATGGATCGGCGATCCGCAAGGCCGGCCGCTGTCGCTGTCGCTGCCGTTCA
CGCCGGGCAACCAGCCGTATCGCGGTCGGCTCGTCAGCGATTATTTCGACAACCTGCTGCCCGACAGCGAGCCGATCCGC
CGGCGAATCGCGATGCGCTACCGCACGGGCGGCACGTCCGCGTTCGCGCTGCTCGCGACGCTCGGCCGCGATTGCGTCGG
CGCGCTGCAGATGCTGCCGCCCGACGAAGCGCCGGACGACATCGAACGCATCCGCGGCCACGCGCTCGCCGACGCGGACA
TCGCGCGCCTGCTGCGCGAAGTCACGTCCGCGCCGCAGGCCGGCCGGCACGCGCCGCTCGACGATCTGCGCCTGTCGATC
GCCGGCGCGCAGGAGAAGACCGCGCTGCTGCGCCACCGCGGCCGCTGGCTGCTGCCCGAAGGGAGCACGCCGACCACGCA
CATCCTGAAGCTGCCGCTCGGGCTCGTCGGCAACCGGCGCGCCGACATGCGCACGTCGGTCGAGAACGAATGGCTGTGCG
CGCGGATCGTCGCCGCGTACGGGTTGCCCGTCGCGCGCTGCGACATCGCGCAGTTCGACGATCAGAAAGCGCTCGTCGTC
GAGCGCTTCGACCGCCGGCCGTCGCGCGACGCGCGCTGGCTCCTGCGGCTGCCGCAGGAAGACATGTGCCAGGCAACCGG
CACGTCCGCGCTCGACAAATATCAGGCCGACGGCGGCCCCGGCATCGAGACGATCATGGAAGTGCTCGCCGGCTCCGAAC
ACGCGCGGGACGACCGCCGCGCGTTCTTCGCGGCGCAGATCGTGTTCTGGCTGCTCGCCGCGACCGACGGCCACGCGAAG
AACTTCAGCATCGCGCACCTGCCCGGCAACCGCTACCGTTCGACACCGCTTTACGACGTGCTGTCCGCGCATCCGGTCAT
CGGCCGGGGCGCGAACCAGTTGCCCGCGCAGCGCGCGCGGCTCGCGATGGGCGTGCGCGGCAAGCACATCCACTATCCGC
TGCACCAGATCCGGCGGCGGCACTGGATCGCGCAGGGCCAGCGCGTCGGCTTCGCGCCCGCCGACGTCGACGCGCTGATC
GACACGCTGACCGCGCGCACCGCGGACGTCGTCGACGCGGTGTCGGCGCGGCTGCCGCGCGATTTTCCGCGCGACGTCGC
CGATGCGATCTTCAGCGGAATGCTCGGCCTGAGCGCAAGGCTCGCCGGCGACGCGGCCGCGCGCGCGCCATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14306.65 Da
• Isoelectric Point: 11.5709

>AT120592 WP_131116773.1 NZ_CP037971:2088011-2088415 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTVAGARRAVPPASAPASAPAPSAAGRPPRPARKRAAPKKREDW

Nucleotide

• Length: 405 bp

>AT120592 NZ_CP037971:2088011-2088415 [Burkholderia pseudomallei]
ATGGCCATCCTCATCGAGCACGAGATCAAGACACTCGGCCAGCTGCGGCCGATTCTGCGCGGCTTCCGCAAATCGGCCGG
ATTGACGCAGGCGATGCTCGCGAGCCGCCTCGGCGTCACGCAGCAGACCTACGCGCAGTTCGAGGCGAACCCGGCGTCGG
CGAGCGTCGAGCGGCTGTTCAAGGTGCTGCGCGCGCTCGACATCGAACTCACGCTCACGCTCACGCAGGTCTACGCCGCG
CCCGCGGGCAAGGACAAGGGCGAGGTTGCGAAGACGGTCGCAGGCGCGCGACGTGCCGTGCCGCCCGCGTCCGCGCCCGC
GTCCGCGCCCGCTCCGAGCGCCGCCGGGCGCCCGCCCCGCCCCGCCCGCAAGCGCGCCGCCCCGAAAAAGCGGGAGGACT
GGTGA

Structures

Toxin

• AlphaFold DB: A0A3N4FSX2