Detailed information of TA system TA120590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 511774..512215
• Replicon: chromosome
• Accession: NZ_CP037971
• Organism: Burkholderia pseudomallei strain BPs133 isolate Melioidosis patient

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: E2R25_RS02735
• Protein ID: WP_009939402.1
• Coordinates: 512042..512215 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: E2R25_RS02730
• Protein ID: WP_004525420.1
• Coordinates: 511774..512058 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2R25_RS02690	506869..507189	+	321	WP_004196029.1	DUF4212 domain-containing protein	-
E2R25_RS02695	507183..509198	+	2016	WP_004525415.1	cation acetate symporter	-
E2R25_RS36235	509318..509866	-	549	WP_166682744.1	toprim domain-containing protein	-
E2R25_RS36240	509764..510294	-	531	WP_166682759.1	PriCT-2 domain-containing protein	-
E2R25_RS02710	510542..510637	+	96	Protein_420	recombinase family protein	-
E2R25_RS02715	510721..511128	-	408	Protein_421	DDE-type integrase/transposase/recombinase	-
E2R25_RS02730	511774..512058	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
E2R25_RS02735	512042..512215	-	174	WP_009939402.1	BrnT family toxin	Toxin
E2R25_RS02740	512883..513158	+	276	WP_004552388.1	bacteriophage protein Gp49	-
E2R25_RS02745	513168..513299	+	132	WP_009923452.1	bacteriophage protein Gp48	-
E2R25_RS36245	513489..513707	+	219	WP_004552392.1	hypothetical protein	-
E2R25_RS36250	513909..514226	+	318	WP_166682741.1	hypothetical protein	-
E2R25_RS02765	514223..514996	+	774	Protein_428	prohibitin family protein	-
E2R25_RS37085	514994..515398	+	405	Protein_429	DDE-type integrase/transposase/recombinase	-
E2R25_RS02770	515361..515789	+	429	Protein_430	IS110 family transposase	-
E2R25_RS02775	516016..516132	-	117	Protein_431	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	507183..521438	14255
	-	inside	IScluster/Tn	-	-	510721..521438	10717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T120590 WP_009939402.1 NZ_CP037971:c512215-512042 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T120590 NZ_CP037971:c512215-512042 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT120590 WP_004525420.1 NZ_CP037971:c512058-511774 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT120590 NZ_CP037971:c512058-511774 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8