Detailed information of TA system TA120585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 511407..511848
• Replicon: chromosome
• Accession: NZ_CP037969
• Organism: Burkholderia pseudomallei strain BPs123

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: E2R23_RS02670
• Protein ID: WP_009939402.1
• Coordinates: 511675..511848 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: E2R23_RS02665
• Protein ID: WP_004525420.1
• Coordinates: 511407..511691 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2R23_RS02635	507750..508070	+	321	WP_004196029.1	DUF4212 domain-containing protein	-
E2R23_RS02640	508064..510079	+	2016	WP_024431205.1	cation acetate symporter	-
E2R23_RS02650	510354..510761	-	408	Protein_413	DDE-type integrase/transposase/recombinase	-
E2R23_RS02665	511407..511691	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
E2R23_RS02670	511675..511848	-	174	WP_009939402.1	BrnT family toxin	Toxin
E2R23_RS02675	512516..512791	+	276	WP_004552388.1	bacteriophage protein Gp49	-
E2R23_RS02680	512801..512932	+	132	WP_009923452.1	bacteriophage protein Gp48	-
E2R23_RS36335	513120..513338	+	219	WP_004552392.1	hypothetical protein	-
E2R23_RS02695	513709..513858	+	150	WP_004537646.1	bacteriophage protein Gp44	-
E2R23_RS02700	513855..514628	+	774	Protein_420	prohibitin family protein	-
E2R23_RS37175	514626..515030	+	405	Protein_421	DDE-type integrase/transposase/recombinase	-
E2R23_RS02705	514993..515421	+	429	Protein_422	IS110 family transposase	-
E2R23_RS02710	515648..515764	-	117	Protein_423	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	508064..521072	13008
	-	inside	IScluster/Tn	-	-	510354..521072	10718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T120585 WP_009939402.1 NZ_CP037969:c511848-511675 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T120585 NZ_CP037969:c511848-511675 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT120585 WP_004525420.1 NZ_CP037969:c511691-511407 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT120585 NZ_CP037969:c511691-511407 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8