Detailed information of TA system TA120584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 52387..53123
• Replicon: plasmid p1_020135
• Accession: NZ_CP037966
• Organism: Klebsiella pneumoniae strain SCKP020135

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: CLQ43_RS27395
• Protein ID: WP_003026803.1
• Coordinates: 52641..53123 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: CLQ43_RS27390
• Protein ID: WP_003026799.1
• Coordinates: 52387..52653 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ43_RS27340	47661..48011	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
CLQ43_RS27345	48369..48638	+	270	WP_004152102.1	hypothetical protein	-
CLQ43_RS27350	48626..49201	+	576	WP_105446397.1	hypothetical protein	-
CLQ43_RS27355	49232..49726	+	495	WP_044816180.1	hypothetical protein	-
CLQ43_RS27360	49770..50138	+	369	WP_004152105.1	hypothetical protein	-
CLQ43_RS27365	50172..50375	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
CLQ43_RS27370	50424..50681	+	258	WP_017899889.1	hypothetical protein	-
CLQ43_RS27375	50898..51878	+	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
CLQ43_RS27385	52002..52211	+	210	WP_065896101.1	hypothetical protein	-
CLQ43_RS27390	52387..52653	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
CLQ43_RS27395	52641..53123	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
CLQ43_RS27400	53324..54727	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
CLQ43_RS27405	54756..55388	-	633	WP_001567369.1	hypothetical protein	-
CLQ43_RS27410	55618..56964	+	1347	WP_020314316.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..179816	179816
	-	inside	IScluster/Tn	-	-	50898..71416	20518

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T120584 WP_003026803.1 NZ_CP037966:52641-53123 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T120584 NZ_CP037966:52641-53123 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT120584 WP_003026799.1 NZ_CP037966:52387-52653 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT120584 NZ_CP037966:52387-52653 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66