Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 6767..7292 Replicon plasmid pMCR8_020135
Accession NZ_CP037964
Organism Klebsiella pneumoniae strain SCKP020135

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag CLQ43_RS25840 Protein ID WP_013023785.1
Coordinates 6987..7292 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag CLQ43_RS25835 Protein ID WP_001568025.1
Coordinates 6767..6985 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ43_RS25800 1923..2702 + 780 WP_013023780.1 hypothetical protein -
CLQ43_RS25805 2909..4501 + 1593 WP_015344964.1 hypothetical protein -
CLQ43_RS25810 4535..5662 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
CLQ43_RS25815 5659..5949 + 291 WP_013023783.1 hypothetical protein -
CLQ43_RS25820 5939..6136 - 198 WP_017899886.1 hypothetical protein -
CLQ43_RS25835 6767..6985 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CLQ43_RS25840 6987..7292 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
CLQ43_RS25845 7461..7856 + 396 WP_017899885.1 hypothetical protein -
CLQ43_RS25850 7883..8197 + 315 WP_053389906.1 hypothetical protein -
CLQ43_RS25855 8208..9224 + 1017 WP_017899884.1 hypothetical protein -
CLQ43_RS25860 9422..10216 + 795 WP_004197635.1 site-specific integrase -
CLQ43_RS25865 10680..10859 - 180 WP_006788217.1 hypothetical protein -
CLQ43_RS25870 10979..11605 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / mcr-8 / aph(3')-Ia / aac(3)-IId / mph(A) / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr - 1..131811 131811


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T120583 WP_013023785.1 NZ_CP037964:6987-7292 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T120583 NZ_CP037964:6987-7292 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT120583 WP_001568025.1 NZ_CP037964:6767-6985 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT120583 NZ_CP037964:6767-6985 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References