Detailed information of TA system TA120565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2564006..2564571
• Replicon: chromosome
• Accession: NZ_CP037961
• Organism: Lactiplantibacillus plantarum strain NCIMB8826

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F9URY0
• Locus tag: E2P15_RS12290
• Protein ID: WP_011101950.1
• Coordinates: 2564006..2564353 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: F9URY1
• Locus tag: E2P15_RS12295
• Protein ID: WP_011101951.1
• Coordinates: 2564353..2564571 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2P15_RS12260 (E2P15_12595)	2559248..2560105	-	858	WP_011101946.1	SDR family oxidoreductase	-
E2P15_RS12265 (E2P15_12600)	2560546..2560767	-	222	WP_011101947.1	hypothetical protein	-
E2P15_RS12270 (E2P15_12605)	2561023..2561466	-	444	WP_003642165.1	universal stress protein	-
E2P15_RS12275 (E2P15_12610)	2561538..2562107	-	570	WP_003642166.1	sugar O-acetyltransferase	-
E2P15_RS12280 (E2P15_12615)	2562204..2562887	+	684	WP_011101948.1	YjjG family noncanonical pyrimidine nucleotidase	-
E2P15_RS12285 (E2P15_12620)	2563037..2563552	-	516	WP_011101949.1	hypothetical protein	-
E2P15_RS12290 (E2P15_12625)	2564006..2564353	-	348	WP_011101950.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E2P15_RS12295 (E2P15_12630)	2564353..2564571	-	219	WP_011101951.1	hypothetical protein	Antitoxin
E2P15_RS12300 (E2P15_12635)	2565335..2565763	-	429	WP_223843853.1	hypothetical protein	-
E2P15_RS12305 (E2P15_12640)	2566701..2567294	-	594	WP_011101953.1	NAD(P)-binding domain-containing protein	-
E2P15_RS12310 (E2P15_12645)	2567443..2568615	-	1173	WP_011101954.1	MalY/PatB family protein	-
E2P15_RS12315 (E2P15_12650)	2568605..2569471	-	867	WP_011101955.1	DUF3737 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13274.06 Da
• Isoelectric Point: 9.5016

>T120565 WP_011101950.1 NZ_CP037961:c2564353-2564006 [Lactiplantibacillus plantarum]
MTYLPKQKDIIWIDFDPQRGREIKKRRPAVVLSSNLYTQNTGFVIVSPITSTMRDLPGYFSLNGYNTHGQIAAAQIYSFD
ATPRAGRSITYIETMRSADFYHVAQTVYYNFDFPF

Nucleotide

• Length: 348 bp

>T120565 NZ_CP037961:c2564353-2564006 [Lactiplantibacillus plantarum]
ATGACTTATTTACCTAAACAAAAAGACATCATCTGGATTGATTTTGACCCACAACGTGGCCGCGAAATAAAGAAACGTCG
GCCAGCTGTAGTTCTTAGTAGTAATCTCTACACTCAGAACACCGGGTTTGTAATTGTTTCACCAATTACGTCTACAATGC
GTGATTTGCCCGGCTACTTTAGTCTTAATGGTTACAATACCCACGGACAAATTGCTGCTGCCCAAATTTATTCGTTTGAT
GCGACCCCCAGAGCAGGACGGAGTATCACATACATCGAAACCATGCGCAGTGCCGATTTTTATCACGTCGCGCAAACGGT
GTATTACAACTTCGACTTTCCATTCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8163.27 Da
• Isoelectric Point: 8.5318

>AT120565 WP_011101951.1 NZ_CP037961:c2564571-2564353 [Lactiplantibacillus plantarum]
MIEVKTVSRGNSLALSLPKDGRFKKGQRWLLIPSSDGESYTLVPRIENPYAGSKPKRPMTEAWSDVDWNEVE

Nucleotide

• Length: 219 bp

>AT120565 NZ_CP037961:c2564571-2564353 [Lactiplantibacillus plantarum]
ATGATTGAGGTAAAGACAGTTAGTCGTGGTAATTCATTGGCATTAAGTCTACCTAAAGACGGTCGTTTTAAGAAAGGACA
ACGATGGTTACTCATCCCATCAAGTGATGGTGAGTCATATACACTCGTACCACGTATCGAGAATCCTTATGCCGGCTCCA
AGCCGAAACGGCCAATGACTGAAGCATGGTCCGATGTCGACTGGAATGAGGTCGAGTAA

Structures

Toxin

• AlphaFold DB: F9URY0

Antitoxin

• AlphaFold DB: F9URY1