Detailed information of TA system TA120543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4487049..4487639
• Replicon: chromosome
• Accession: NZ_CP037950
• Organism: Erwinia sp. QL-Z3

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: E2F51_RS21575
• Protein ID: WP_134824528.1
• Coordinates: 4487049..4487381 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D8MUA0
• Locus tag: E2F51_RS21580
• Protein ID: WP_013202892.1
• Coordinates: 4487382..4487639 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2F51_RS21555	4482678..4484783	-	2106	WP_134824527.1	elongation factor G	-
E2F51_RS21560	4485005..4485313	+	309	WP_013202889.1	helix-turn-helix domain-containing protein	-
E2F51_RS21565	4485506..4486627	-	1122	WP_013202890.1	Gfo/Idh/MocA family oxidoreductase	-
E2F51_RS21575	4487049..4487381	-	333	WP_134824528.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E2F51_RS21580	4487382..4487639	-	258	WP_013202892.1	antitoxin	Antitoxin
E2F51_RS21590	4487900..4488556	-	657	WP_134824530.1	DUF998 domain-containing protein	-
E2F51_RS21595	4488570..4489451	-	882	WP_134824531.1	siderophore-interacting protein	-
E2F51_RS21600	4489604..4490833	-	1230	WP_134824532.1	MFS transporter	-
E2F51_RS21605	4490862..4492298	-	1437	WP_134824533.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (5-85)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12002.67 Da
• Isoelectric Point: 6.4611

>T120543 WP_134824528.1 NZ_CP037950:c4487381-4487049 [Erwinia sp. QL-Z3]
MERGEIWLVSLDPTAGHEQRGTRPVLIVSPAAFNRVTQLPVVVPITRNGNFARTAGFTVSLDGAGTTTTGVIRCDQPRTI
DMSARHGERLERLPDDVVNEVLARLEAILN

Nucleotide

• Length: 333 bp

>T120543 NZ_CP037950:c4487381-4487049 [Erwinia sp. QL-Z3]
ATGGAGAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGACAGCGGGACATGAACAACGTGGGACTCGTCCGGTACTCAT
TGTATCTCCCGCCGCATTTAACCGGGTCACTCAGTTACCTGTCGTCGTTCCCATTACCCGAAATGGAAACTTCGCCCGCA
CAGCGGGGTTTACCGTATCACTGGATGGAGCAGGAACTACAACAACGGGTGTGATTCGCTGCGATCAGCCGAGAACCATT
GATATGAGTGCCCGGCACGGTGAGAGACTGGAACGTCTGCCCGATGATGTTGTTAATGAAGTGCTGGCCAGACTGGAAGC
TATTCTTAATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9435.04 Da
• Isoelectric Point: 4.5982

>AT120543 WP_013202892.1 NZ_CP037950:c4487639-4487382 [Erwinia sp. QL-Z3]
MYITRLKKVGGSVMLAVPPAVLKTLALSTESEVGMTIKNGCLIIEPQKRPRYSLEELLAQCDPQAEMSEEDREWMDTPVA
GKEVL

Nucleotide

• Length: 258 bp

>AT120543 NZ_CP037950:c4487639-4487382 [Erwinia sp. QL-Z3]
ATGTATATCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTAAAAACGTTGGCGTT
GTCGACAGAAAGCGAAGTGGGCATGACCATTAAAAATGGCTGCCTGATCATTGAACCGCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCAGGCTGAAATGAGCGAAGAAGATCGGGAATGGATGGATACGCCTGTGGCA
GGCAAGGAGGTCCTGTAA

Structures

Antitoxin

• AlphaFold DB: D8MUA0