Detailed information of TA system TA120540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2601258..2601886
• Replicon: chromosome
• Accession: NZ_CP037950
• Organism: Erwinia sp. QL-Z3

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D8MP01
• Locus tag: E2F51_RS12585
• Protein ID: WP_013201057.1
• Coordinates: 2601258..2601476 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: D8MP02
• Locus tag: E2F51_RS12590
• Protein ID: WP_013201058.1
• Coordinates: 2601506..2601886 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2F51_RS12555	2597133..2597471	+	339	WP_007886947.1	P-II family nitrogen regulator	-
E2F51_RS12560	2597507..2598796	+	1290	WP_041692221.1	ammonium transporter AmtB	-
E2F51_RS12565	2598885..2599751	-	867	WP_013201054.1	acyl-CoA thioesterase II	-
E2F51_RS12570	2599973..2600530	+	558	WP_134823600.1	YbaY family lipoprotein	-
E2F51_RS12575	2600555..2600866	-	312	WP_013201056.1	MGMT family protein	-
E2F51_RS12585	2601258..2601476	-	219	WP_013201057.1	hemolysin expression modulator Hha	Toxin
E2F51_RS12590	2601506..2601886	-	381	WP_013201058.1	Hha toxicity modulator TomB	Antitoxin
E2F51_RS12595	2602031..2602384	-	354	WP_134823601.1	hypothetical protein	-
E2F51_RS12600	2602800..2603486	+	687	WP_053141862.1	ABC transporter ATP-binding protein	-
E2F51_RS12605	2603489..2604355	+	867	WP_134824865.1	metal ABC transporter permease	-
E2F51_RS12610	2604352..2605230	+	879	WP_134823602.1	metal ABC transporter substrate-binding protein	-
E2F51_RS12615	2605323..2605463	-	141	WP_013201062.1	type B 50S ribosomal protein L36	-
E2F51_RS12620	2605473..2605736	-	264	WP_053141865.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.10 Da
• Isoelectric Point: 9.4825

>T120540 WP_013201057.1 NZ_CP037950:c2601476-2601258 [Erwinia sp. QL-Z3]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 219 bp

>T120540 NZ_CP037950:c2601476-2601258 [Erwinia sp. QL-Z3]
ATGACAGATAAACTTCTTACTAAAACTGATTATCTGATGCGTTTAAGACGGTGTCGGTCAATCGACACCCTCGAGCGCGT
AATCGAAAAAAACAAATACGAATTATCCGATAATGAACTGGCTGTGTTTTATTCTGCGGCTGATCATCGTCTGGCCGAAC
TGACGATGAATAAACTCTACGATAAAATCCCGGTAGCAGTTTGGAAGTTCGTTCGATAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14596.35 Da
• Isoelectric Point: 4.4699

>AT120540 WP_013201058.1 NZ_CP037950:c2601886-2601506 [Erwinia sp. QL-Z3]
MDEYSPKRHDIAQLKFVCENLYDESLATLGDSHHGWVNDPTAAVNLQLNDLIEHIAAFTMNYKIKHLEDSDLISQIDEYL
DDTFMLFSNYGINVQDLQRWQKSAKRLFNIFAEECALVPVQTSHSF

Nucleotide

• Length: 381 bp

>AT120540 NZ_CP037950:c2601886-2601506 [Erwinia sp. QL-Z3]
ATGGACGAATACTCACCAAAAAGGCATGATATCGCCCAGCTGAAATTTGTGTGTGAAAATTTGTACGATGAAAGCCTGGC
GACGTTAGGCGATAGTCATCACGGCTGGGTTAACGATCCTACTGCTGCAGTCAATTTACAGCTCAATGATCTGATTGAGC
ATATTGCTGCCTTCACCATGAATTACAAAATTAAACATCTCGAAGATAGTGACCTGATTAGTCAGATTGATGAATATCTC
GATGATACGTTTATGCTCTTTAGTAATTACGGCATTAATGTTCAGGATTTGCAGCGCTGGCAAAAATCAGCAAAACGCTT
GTTTAACATTTTCGCCGAGGAATGCGCTCTCGTGCCCGTACAGACGAGCCATTCATTTTAG

Structures

Toxin

• AlphaFold DB: D8MP01

Antitoxin

• AlphaFold DB: D8MP02