Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 36173..36754 Replicon plasmid unnamed1
Accession NZ_CP037949
Organism Erwinia sp. QL-Z3

Toxin (Protein)


Gene name mazF Uniprot ID D8MK01
Locus tag E2F51_RS00180 Protein ID WP_013199966.1
Coordinates 36422..36754 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A328T8X3
Locus tag E2F51_RS00175 Protein ID WP_041691825.1
Coordinates 36173..36421 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2F51_RS00150 32229..33200 + 972 WP_134822402.1 ParB/RepB/Spo0J family partition protein -
E2F51_RS23755 33897..35004 + 1108 Protein_26 IS3 family transposase -
E2F51_RS00165 35110..35505 + 396 WP_134822403.1 VOC family protein -
E2F51_RS00175 36173..36421 + 249 WP_041691825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E2F51_RS00180 36422..36754 + 333 WP_013199966.1 endoribonuclease MazF Toxin
E2F51_RS00185 37367..37594 - 228 WP_134822404.1 DNA partition complex ParG -
E2F51_RS00190 37633..38253 - 621 WP_008815938.1 ParA family protein -
E2F51_RS00195 38842..40116 - 1275 WP_134822405.1 translesion error-prone DNA polymerase V subunit UmuC -
E2F51_RS00200 40120..40548 - 429 WP_134822406.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
E2F51_RS00205 40667..40879 + 213 WP_134822407.1 hypothetical protein -
E2F51_RS00210 41161..41358 - 198 WP_134822408.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..149889 149889
- flank IS/Tn - - 33897..34163 266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

Antitoxin PemI-like

(4-81)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11955.70 Da        Isoelectric Point: 7.1057

>T120538 WP_013199966.1 NZ_CP037949:36422-36754 [Erwinia sp. QL-Z3]
MVSRYVPNSGDLIWLDFDPVEGHEQGGHRPAVVLSPFSYNNPVGLLLCVPCTTKAKGYPFEVELSGSRESVALADQVTCV
DWRARKVTKKGTVSPDELAEIRAKAKALIG

Download         Length: 333 bp

>T120538 NZ_CP037949:36422-36754 [Erwinia sp. QL-Z3]
ATGGTTTCACGTTACGTACCAAATTCTGGTGATCTAATCTGGCTGGATTTTGACCCCGTAGAAGGTCACGAACAGGGCGG
CCATCGCCCTGCTGTCGTCCTTAGCCCTTTTTCTTACAATAATCCCGTAGGGTTGCTGCTTTGCGTTCCGTGTACCACTA
AAGCTAAAGGCTATCCATTTGAGGTAGAACTATCCGGTAGCCGCGAAAGTGTTGCTCTTGCCGATCAAGTTACCTGTGTT
GACTGGAGAGCGCGGAAAGTAACAAAAAAAGGCACTGTATCGCCTGACGAACTTGCTGAGATCAGAGCAAAGGCTAAAGC
TCTAATTGGATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8815.28 Da        Isoelectric Point: 5.7630

>AT120538 WP_041691825.1 NZ_CP037949:36173-36421 [Erwinia sp. QL-Z3]
MTTVTLKKWGNSPSIRIPASVMQAAALNVEDKLDLKIDEAGQIVLVPLKSKEYSLDVLLSGITPDNVHGKIDFGSPVGKE
LI

Download         Length: 249 bp

>AT120538 NZ_CP037949:36173-36421 [Erwinia sp. QL-Z3]
ATGACAACAGTAACGCTAAAAAAATGGGGCAATAGCCCATCGATCAGAATACCAGCCAGCGTAATGCAAGCAGCAGCACT
CAATGTCGAAGATAAACTCGATTTGAAAATTGATGAAGCTGGCCAAATTGTTTTAGTACCATTGAAATCTAAAGAGTATT
CACTAGATGTACTGTTATCAGGTATCACACCAGATAACGTTCACGGAAAAATAGATTTTGGTTCGCCAGTTGGGAAGGAA
TTGATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D8MK01

Antitoxin

Source ID Structure
AlphaFold DB A0A328T8X3

References