Detailed information of TA system TA120531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 247..677
• Replicon: plasmid pCFSAN027346-2
• Accession: NZ_CP037947
• Organism: Escherichia coli strain CFSAN027346

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: EKI52_RS29525
• Protein ID: WP_001388374.1
• Coordinates: 513..677 (+)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6Y0EDY2
• Locus tag: EKI52_RS29520
• Protein ID: WP_000079939.1
• Coordinates: 247..516 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI52_RS30260	34..174	+	141	WP_021503378.1	hypothetical protein	-
EKI52_RS29520	247..516	+	270	WP_000079939.1	hypothetical protein	Antitoxin
EKI52_RS29525	513..677	+	165	WP_001388374.1	hypothetical protein	Toxin
EKI52_RS29530	725..1648	+	924	WP_001415483.1	hypothetical protein	-
EKI52_RS29540	2090..2470	+	381	WP_001696636.1	HTH domain-containing protein	-
EKI52_RS29545	2582..2986	+	405	Protein_5	IS91 family transposase	-
EKI52_RS29550	3093..3929	-	837	WP_000480972.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
EKI52_RS29555	3929..4732	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
EKI52_RS29560	4793..5608	-	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / dfrA8 / tet(B)	-	1..73152	73152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6604.73 Da
• Isoelectric Point: 7.1989

>T120531 WP_001388374.1 NZ_CP037947:513-677 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYFGRTHRLTPVLHLYYM

Nucleotide

• Length: 165 bp

>T120531 NZ_CP037947:513-677 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAATACATTGCGGAGCAGAACCTGAT
AGCAGCCATTGAACTGGACGAACGGATTGGTTATTTCGGACGAACGCACCGGCTTACGCCGGTTTTACATCTGTACTACA
TGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10233.54 Da
• Isoelectric Point: 6.7263

>AT120531 WP_000079939.1 NZ_CP037947:247-516 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT120531 NZ_CP037947:247-516 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCACGGCGATGTACTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGAGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6Y0EDY2