Detailed information of TA system TA120530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 58488..59131
• Replicon: plasmid pCFSAN027346-1
• Accession: NZ_CP037946
• Organism: Escherichia coli strain CFSAN027346

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: EKI52_RS29325
• Protein ID: WP_001044768.1
• Coordinates: 58715..59131 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: EKI52_RS29320
• Protein ID: WP_001261287.1
• Coordinates: 58488..58718 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI52_RS29275	54353..55792	+	1440	WP_001213545.1	enterohemolysin T1SS ABC transporter subunit EhxD	-
EKI52_RS29280	55859..56002	+	144	Protein_60	transcriptional regulator	-
EKI52_RS29285	56006..56197	+	192	Protein_61	lipid II-degrading bacteriocin	-
EKI52_RS29290	56205..56558	-	354	WP_000864810.1	colicin M immunity protein	-
EKI52_RS29295	56731..57513	-	783	WP_001164205.1	site-specific integrase	-
EKI52_RS29300	57515..57928	-	414	WP_000465041.1	hypothetical protein	-
EKI52_RS29320	58488..58718	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EKI52_RS29325	58715..59131	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
EKI52_RS29330	59293..59838	-	546	WP_001165114.1	hypothetical protein	-
EKI52_RS29335	59906..61062	+	1157	WP_085948186.1	IS3-like element IS600 family transposase	-
EKI52_RS30255	61103..61252	-	150	WP_000955366.1	hypothetical protein	-
EKI52_RS29340	61867..62727	-	861	WP_000704534.1	alpha/beta hydrolase	-
EKI52_RS29345	62855..63241	+	387	WP_001066949.1	tyrosine-type recombinase/integrase	-
EKI52_RS29350	63295..63969	+	675	WP_001341423.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	espP / hlyC / hlyA / hlyB / hlyD / toxB / toxB	1..96016	96016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T120530 WP_001044768.1 NZ_CP037946:58715-59131 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T120530 NZ_CP037946:58715-59131 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT120530 WP_001261287.1 NZ_CP037946:58488-58718 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT120530 NZ_CP037946:58488-58718 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4