Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 5231353..5231824 Replicon chromosome
Accession NZ_CP037945
Organism Escherichia coli strain CFSAN027346

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag EKI52_RS26940 Protein ID WP_001448501.1
Coordinates 5231353..5231631 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag EKI52_RS26945 Protein ID WP_001302048.1
Coordinates 5231633..5231824 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI52_RS26915 5227003..5229654 - 2652 WP_000105101.1 exodeoxyribonuclease VIII -
EKI52_RS26920 5229753..5230028 - 276 WP_001307773.1 hypothetical protein -
EKI52_RS26925 5230102..5230278 - 177 WP_000245534.1 hypothetical protein -
EKI52_RS26930 5230272..5230493 - 222 WP_000560218.1 killing protein KilR -
EKI52_RS26935 5230914..5231066 - 153 WP_001427316.1 DUF1391 family protein -
EKI52_RS26940 5231353..5231631 - 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EKI52_RS26945 5231633..5231824 - 192 WP_001302048.1 hypothetical protein Antitoxin
EKI52_RS26950 5231845..5232216 - 372 WP_001169687.1 hypothetical protein -
EKI52_RS26955 5232314..5232616 + 303 WP_000172738.1 transcriptional regulator -
EKI52_RS26960 5232613..5233038 + 426 WP_000693943.1 Rha family transcriptional regulator -
EKI52_RS26965 5233061..5234023 + 963 WP_001444941.1 helix-turn-helix domain-containing protein -
EKI52_RS26970 5234030..5234771 + 742 Protein_5135 ATP-binding protein -
EKI52_RS26975 5234797..5235558 + 762 WP_000450872.1 DUF1627 domain-containing protein -
EKI52_RS26980 5235591..5235872 + 282 WP_000603384.1 DNA-binding protein -
EKI52_RS26985 5235869..5236096 + 228 WP_000699809.1 hypothetical protein -
EKI52_RS26990 5236089..5236400 + 312 WP_001289673.1 hypothetical protein -
EKI52_RS26995 5236528..5236746 + 219 WP_000683609.1 DUF4014 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 5216565..5289464 72899


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T120529 WP_001448501.1 NZ_CP037945:c5231631-5231353 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T120529 NZ_CP037945:c5231631-5231353 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT120529 WP_001302048.1 NZ_CP037945:c5231824-5231633 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT120529 NZ_CP037945:c5231824-5231633 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References