Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4974473..4975308 Replicon chromosome
Accession NZ_CP037945
Organism Escherichia coli strain CFSAN027346

Toxin (Protein)


Gene name yeeV Uniprot ID A0A136X5A2
Locus tag EKI52_RS25570 Protein ID WP_000854757.1
Coordinates 4974473..4974850 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag EKI52_RS25575 Protein ID WP_001295723.1
Coordinates 4974940..4975308 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI52_RS25535 4970664..4971722 + 1059 WP_001200887.1 FUSC family protein -
EKI52_RS25540 4971894..4972223 + 330 WP_000450409.1 DUF496 family protein -
EKI52_RS25545 4972324..4972506 - 183 WP_024167529.1 ethanolamine utilization protein -
EKI52_RS30370 4972960..4973331 - 372 WP_001295631.1 IS110 family transposase -
EKI52_RS25560 4974147..4974260 - 114 WP_001161660.1 DUF957 domain-containing protein -
EKI52_RS25565 4974273..4974476 - 204 WP_000761705.1 hypothetical protein -
EKI52_RS25570 4974473..4974850 - 378 WP_000854757.1 TA system toxin CbtA family protein Toxin
EKI52_RS25575 4974940..4975308 - 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EKI52_RS25580 4975471..4975692 - 222 WP_000692323.1 DUF987 domain-containing protein -
EKI52_RS25585 4975761..4976237 - 477 WP_001186725.1 RadC family protein -
EKI52_RS25590 4976253..4976738 - 486 WP_000849582.1 antirestriction protein -
EKI52_RS25595 4976793..4977611 - 819 WP_001234620.1 DUF945 domain-containing protein -
EKI52_RS25605 4977711..4977944 - 234 WP_001119729.1 DUF905 family protein -
EKI52_RS25610 4978023..4978364 - 342 WP_072097794.1 hypothetical protein -
EKI52_RS25615 4978453..4978857 + 405 WP_000839179.1 IS66 family insertion sequence hypothetical protein -
EKI52_RS25620 4978854..4979201 + 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14153.18 Da        Isoelectric Point: 7.3249

>T120516 WP_000854757.1 NZ_CP037945:c4974850-4974473 [Escherichia coli]
MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T120516 NZ_CP037945:c4974850-4974473 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCTCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAACAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT120516 WP_001295723.1 NZ_CP037945:c4975308-4974940 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT120516 NZ_CP037945:c4975308-4974940 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A136X5A2

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References