Detailed information of TA system TA120506

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2245364..2246196
• Replicon: chromosome
• Accession: NZ_CP037945
• Organism: Escherichia coli strain CFSAN027346

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: EKI52_RS11965
• Protein ID: WP_000854753.1
• Coordinates: 2245364..2245738 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: EKI52_RS11970
• Protein ID: WP_001315620.1
• Coordinates: 2245828..2246196 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI52_RS11915	2240475..2241455	+	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
EKI52_RS11920	2241463..2242113	-	651	WP_001037966.1	HNH endonuclease	-
EKI52_RS30115	2243166..2243318	-	153	WP_001280445.1	hypothetical protein	-
EKI52_RS11945	2243403..2243600	-	198	WP_000839281.1	DUF957 domain-containing protein	-
EKI52_RS11950	2243612..2244046	-	435	WP_096955184.1	hypothetical protein	-
EKI52_RS11955	2244138..2245294	+	1157	WP_085948186.1	IS3-like element IS600 family transposase	-
EKI52_RS11965	2245364..2245738	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
EKI52_RS11970	2245828..2246196	-	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EKI52_RS11975	2246359..2246580	-	222	WP_000692345.1	DUF987 domain-containing protein	-
EKI52_RS11980	2246643..2247119	-	477	WP_001186774.1	RadC family protein	-
EKI52_RS11985	2247135..2247620	-	486	WP_000849588.1	antirestriction protein	-
EKI52_RS11990	2247675..2248493	-	819	WP_001234622.1	DUF945 domain-containing protein	-
EKI52_RS12000	2248593..2248826	-	234	WP_001119729.1	DUF905 family protein	-
EKI52_RS12005	2248905..2249360	-	456	WP_133342163.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimE / fimB	2235436..2247620	12184

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T120506 WP_000854753.1 NZ_CP037945:c2245738-2245364 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T120506 NZ_CP037945:c2245738-2245364 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT120506 WP_001315620.1 NZ_CP037945:c2246196-2245828 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT120506 NZ_CP037945:c2246196-2245828 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5