Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1593916..1594534 Replicon chromosome
Accession NZ_CP037945
Organism Escherichia coli strain CFSAN027346

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EKI52_RS08810 Protein ID WP_001291435.1
Coordinates 1594316..1594534 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EKI52_RS08805 Protein ID WP_000344800.1
Coordinates 1593916..1594290 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI52_RS08795 1589005..1590198 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EKI52_RS08800 1590221..1593370 + 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
EKI52_RS08805 1593916..1594290 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EKI52_RS08810 1594316..1594534 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EKI52_RS08815 1594706..1595257 + 552 WP_000102564.1 maltose O-acetyltransferase -
EKI52_RS08820 1595373..1595843 + 471 WP_000136192.1 YlaC family protein -
EKI52_RS08825 1596007..1597557 + 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EKI52_RS08830 1597599..1597952 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EKI52_RS08840 1598330..1598641 + 312 WP_000409911.1 MGMT family protein -
EKI52_RS08845 1598672..1599244 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T120503 WP_001291435.1 NZ_CP037945:1594316-1594534 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T120503 NZ_CP037945:1594316-1594534 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT120503 WP_000344800.1 NZ_CP037945:1593916-1594290 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT120503 NZ_CP037945:1593916-1594290 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References