Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 131640..132278 Replicon chromosome
Accession NZ_CP037945
Organism Escherichia coli strain CFSAN027346

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EKI52_RS00590 Protein ID WP_000813794.1
Coordinates 132102..132278 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EKI52_RS00585 Protein ID WP_001270286.1
Coordinates 131640..132056 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI52_RS00565 126793..127734 - 942 WP_001251332.1 ABC transporter permease -
EKI52_RS00570 127735..128748 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
EKI52_RS00575 128766..129911 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
EKI52_RS00580 130156..131561 - 1406 Protein_116 PLP-dependent aminotransferase family protein -
EKI52_RS00585 131640..132056 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EKI52_RS00590 132102..132278 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EKI52_RS00595 132500..132730 + 231 WP_000494244.1 YncJ family protein -
EKI52_RS00600 132822..134783 - 1962 WP_001341357.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EKI52_RS00605 134856..135392 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
EKI52_RS00610 135445..136659 + 1215 WP_001307190.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 136699..137847 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T120500 WP_000813794.1 NZ_CP037945:c132278-132102 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T120500 NZ_CP037945:c132278-132102 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT120500 WP_001270286.1 NZ_CP037945:c132056-131640 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT120500 NZ_CP037945:c132056-131640 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References