Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 16963..17606 Replicon plasmid pCFSAN027343
Accession NZ_CP037944
Organism Escherichia coli strain CFSAN027343

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag EKI57_RS29675 Protein ID WP_001044768.1
Coordinates 16963..17379 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag EKI57_RS29680 Protein ID WP_001261287.1
Coordinates 17376..17606 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI57_RS29650 13052..13399 - 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
EKI57_RS29655 13396..14070 - 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
EKI57_RS29660 14124..14510 - 387 WP_001066949.1 tyrosine-type recombinase/integrase -
EKI57_RS29665 14638..15498 + 861 WP_000704534.1 alpha/beta hydrolase -
EKI57_RS29670 16256..16801 + 546 WP_001165114.1 hypothetical protein -
EKI57_RS29675 16963..17379 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
EKI57_RS29680 17376..17606 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EKI57_RS29700 18166..18579 + 414 WP_000465041.1 hypothetical protein -
EKI57_RS29705 18581..19363 + 783 WP_001164205.1 site-specific integrase -
EKI57_RS29710 19536..19889 + 354 WP_000864810.1 colicin M immunity protein -
EKI57_RS29715 19897..20088 - 192 Protein_16 lipid II-degrading bacteriocin -
EKI57_RS29720 20092..20235 - 144 Protein_17 transcriptional regulator -
EKI57_RS29725 20302..21741 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - toxB / hlyD / hlyB / hlyA / hlyC / espP 1..88848 88848


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T120498 WP_001044768.1 NZ_CP037944:c17379-16963 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T120498 NZ_CP037944:c17379-16963 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT120498 WP_001261287.1 NZ_CP037944:c17606-17376 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT120498 NZ_CP037944:c17606-17376 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References