Detailed information of TA system TA120497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 5644167..5644645
• Replicon: chromosome
• Accession: NZ_CP037943
• Organism: Escherichia coli strain CFSAN027343

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: EKI57_RS29280
• Protein ID: WP_001303876.1
• Coordinates: 5644167..5644454 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: EKI57_RS29285
• Protein ID: WP_000536233.1
• Coordinates: 5644454..5644645 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI57_RS29245	5639317..5641788	-	2472	WP_032278809.1	exonuclease	-
EKI57_RS29250	5641882..5642073	-	192	WP_001098307.1	DUF1482 family protein	-
EKI57_RS29255	5642070..5642258	-	189	WP_000413705.1	cell division inhibition protein DicB	-
EKI57_RS29265	5642832..5643017	+	186	WP_001133046.1	hypothetical protein	-
EKI57_RS29270	5643204..5643593	-	390	WP_000394511.1	hypothetical protein	-
EKI57_RS29275	5643735..5643890	-	156	WP_000379575.1	DUF1391 family protein	-
EKI57_RS29280	5644167..5644454	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EKI57_RS29285	5644454..5644645	-	192	WP_000536233.1	hypothetical protein	Antitoxin
EKI57_RS29290	5644673..5645074	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
EKI57_RS29295	5645183..5645455	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
EKI57_RS29300	5645439..5645864	+	426	WP_000693855.1	Rha family transcriptional regulator	-
EKI57_RS29305	5646070..5646525	-	456	WP_000273724.1	hypothetical protein	-
EKI57_RS29310	5646604..5647695	+	1092	WP_001205823.1	hypothetical protein	-
EKI57_RS29315	5647702..5648448	+	747	WP_000788751.1	ATP-binding protein	-
EKI57_RS29320	5648470..5649240	+	771	WP_000451007.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5618169..5656100	37931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T120497 WP_001303876.1 NZ_CP037943:c5644454-5644167 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T120497 NZ_CP037943:c5644454-5644167 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT120497 WP_000536233.1 NZ_CP037943:c5644645-5644454 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT120497 NZ_CP037943:c5644645-5644454 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA