Detailed information of TA system TA120490

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3980940..3981772
• Replicon: chromosome
• Accession: NZ_CP037943
• Organism: Escherichia coli strain CFSAN027343

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: EKI57_RS20515
• Protein ID: WP_000854753.1
• Coordinates: 3981398..3981772 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: EKI57_RS20510
• Protein ID: WP_001315620.1
• Coordinates: 3980940..3981308 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI57_RS20475	3977776..3978231	+	456	WP_000581504.1	hypothetical protein	-
EKI57_RS20480	3978310..3978543	+	234	WP_001119729.1	DUF905 family protein	-
EKI57_RS20490	3978643..3979461	+	819	WP_001234622.1	DUF945 domain-containing protein	-
EKI57_RS20495	3979516..3980001	+	486	WP_000849588.1	antirestriction protein	-
EKI57_RS20500	3980017..3980493	+	477	WP_001186774.1	RadC family protein	-
EKI57_RS20505	3980556..3980777	+	222	WP_000692345.1	DUF987 domain-containing protein	-
EKI57_RS20510	3980940..3981308	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EKI57_RS20515	3981398..3981772	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
EKI57_RS20520	3981769..3982257	+	489	WP_000777547.1	hypothetical protein	-
EKI57_RS20525	3982269..3982466	+	198	WP_000839281.1	DUF957 domain-containing protein	-
EKI57_RS30275	3982551..3982703	+	153	WP_001280445.1	hypothetical protein	-
EKI57_RS20550	3983748..3984398	+	651	WP_001037966.1	HNH endonuclease	-
EKI57_RS20555	3984406..3985386	-	981	WP_000991416.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
EKI57_RS20560	3985451..3986557	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T120490 WP_000854753.1 NZ_CP037943:3981398-3981772 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T120490 NZ_CP037943:3981398-3981772 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT120490 WP_001315620.1 NZ_CP037943:3980940-3981308 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT120490 NZ_CP037943:3980940-3981308 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5