Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 976358..976829 Replicon chromosome
Accession NZ_CP037943
Organism Escherichia coli strain CFSAN027343

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag EKI57_RS05450 Protein ID WP_001303511.1
Coordinates 976551..976829 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag EKI57_RS05445 Protein ID WP_001302048.1
Coordinates 976358..976549 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI57_RS05395 971435..971653 - 219 WP_000683609.1 DUF4014 family protein -
EKI57_RS05400 971781..972092 - 312 WP_001289673.1 hypothetical protein -
EKI57_RS05405 972085..972312 - 228 WP_000699809.1 hypothetical protein -
EKI57_RS05410 972309..972590 - 282 WP_000603384.1 DNA-binding protein -
EKI57_RS05415 972623..973385 - 763 Protein_1045 DUF1627 domain-containing protein -
EKI57_RS05420 973411..974152 - 742 Protein_1046 ATP-binding protein -
EKI57_RS05425 974159..975121 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
EKI57_RS05430 975144..975569 - 426 WP_000693943.1 Rha family transcriptional regulator -
EKI57_RS05435 975566..975868 - 303 WP_000172738.1 transcriptional regulator -
EKI57_RS05440 975966..976337 + 372 WP_001169687.1 hypothetical protein -
EKI57_RS05445 976358..976549 + 192 WP_001302048.1 hypothetical protein Antitoxin
EKI57_RS05450 976551..976829 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EKI57_RS05455 977116..977268 + 153 WP_001427316.1 DUF1391 family protein -
EKI57_RS05460 977689..977910 + 222 WP_000560218.1 killing protein KilR -
EKI57_RS05465 977904..978080 + 177 WP_000245534.1 hypothetical protein -
EKI57_RS05470 978154..978429 + 276 WP_001307773.1 hypothetical protein -
EKI57_RS05475 978528..981179 + 2652 WP_133394881.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 921297..991618 70321


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T120471 WP_001303511.1 NZ_CP037943:976551-976829 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T120471 NZ_CP037943:976551-976829 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT120471 WP_001302048.1 NZ_CP037943:976358-976549 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT120471 NZ_CP037943:976358-976549 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References