Detailed information of TA system TA120462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 115294..115841
• Replicon: plasmid pCFSAN027350
• Accession: NZ_CP037942
• Organism: Escherichia coli strain CFSAN027350

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: EKI55_RS28425
• Protein ID: WP_000969996.1
• Coordinates: 115560..115841 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: EKI55_RS28420
• Protein ID: WP_032209716.1
• Coordinates: 115294..115563 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI55_RS28365	110927..111139	+	213	WP_032345400.1	hypothetical protein	-
EKI55_RS28375	111383..111844	+	462	WP_001233850.1	thermonuclease family protein	-
EKI55_RS28380	111890..112099	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
EKI55_RS28385	112137..112727	+	591	WP_000766812.1	DUF2726 domain-containing protein	-
EKI55_RS28390	112967..113221	+	255	WP_000083830.1	replication regulatory protein RepA	-
EKI55_RS28400	113457..113531	+	75	WP_001370046.1	RepA leader peptide Tap	-
EKI55_RS28405	113524..114381	+	858	WP_032345402.1	incFII family plasmid replication initiator RepA	-
EKI55_RS28420	115294..115563	+	270	WP_032209716.1	hypothetical protein	Antitoxin
EKI55_RS28425	115560..115841	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EKI55_RS28435	116355..117563	-	1209	WP_000198533.1	IS91 family transposase	-
EKI55_RS28440	117544..117816	-	273	WP_000019163.1	hypothetical protein	-
EKI55_RS28450	118068..119276	-	1209	WP_000198533.1	IS91 family transposase	-
EKI55_RS28455	119257..119529	-	273	WP_000019163.1	hypothetical protein	-
EKI55_RS28470	120104..120691	+	588	WP_032345404.1	serine acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / toxB / hlyD / hlyB / hlyA / hlyC	1..157534	157534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T120462 WP_000969996.1 NZ_CP037942:115560-115841 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T120462 NZ_CP037942:115560-115841 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10208.57 Da
• Isoelectric Point: 8.8735

>AT120462 WP_032209716.1 NZ_CP037942:115294-115563 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQIAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT120462 NZ_CP037942:115294-115563 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCAGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGATTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3