Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94227..94870 Replicon plasmid pCFSAN027350
Accession NZ_CP037942
Organism Escherichia coli strain CFSAN027350

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag EKI55_RS28265 Protein ID WP_001044768.1
Coordinates 94227..94643 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag EKI55_RS28270 Protein ID WP_001261287.1
Coordinates 94640..94870 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI55_RS28240 89946..90293 - 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
EKI55_RS28245 90293..90970 - 678 WP_100699708.1 IS66-like element accessory protein TnpA -
EKI55_RS28250 91020..92477 + 1458 Protein_91 IS66-like element ISEc8 family transposase -
EKI55_RS28255 92657..93507 - 851 Protein_92 IS3 family transposase -
EKI55_RS28260 93565..94065 + 501 WP_072279305.1 hypothetical protein -
EKI55_RS28265 94227..94643 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
EKI55_RS28270 94640..94870 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EKI55_RS28280 95431..95844 + 414 WP_000465041.1 hypothetical protein -
EKI55_RS28285 95846..96628 + 783 WP_001164190.1 site-specific integrase -
EKI55_RS28290 96800..97153 + 354 WP_000864811.1 colicin M immunity protein -
EKI55_RS28295 97203..97364 - 162 Protein_99 lipid II-degrading bacteriocin -
EKI55_RS28300 97394..97489 - 96 Protein_100 transcriptional regulator -
EKI55_RS28305 97556..98995 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / toxB / hlyD / hlyB / hlyA / hlyC 1..157534 157534


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T120461 WP_001044768.1 NZ_CP037942:c94643-94227 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T120461 NZ_CP037942:c94643-94227 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT120461 WP_001261287.1 NZ_CP037942:c94870-94640 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT120461 NZ_CP037942:c94870-94640 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAATCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References