Detailed information of TA system TA120460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 56506..57007
• Replicon: plasmid pCFSAN027350
• Accession: NZ_CP037942
• Organism: Escherichia coli strain CFSAN027350

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A6X6XTU7
• Locus tag: EKI55_RS28070
• Protein ID: WP_000702245.1
• Coordinates: 56747..57007 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: EKI55_RS28065
• Protein ID: WP_001132407.1
• Coordinates: 56506..56757 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI55_RS28020	52157..53370	+	1214	WP_165963876.1	IS3 family transposase	-
EKI55_RS28030	53501..53938	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
EKI55_RS28035	53963..54145	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
EKI55_RS28040	54703..54885	+	183	WP_001481829.1	hypothetical protein	-
EKI55_RS28045	54992..55258	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
EKI55_RS28050	55678..55956	-	279	WP_000545937.1	hypothetical protein	-
EKI55_RS28065	56506..56757	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
EKI55_RS28070	56747..57007	-	261	WP_000702245.1	hypothetical protein	Toxin
EKI55_RS28075	57059..57283	-	225	WP_000814381.1	hypothetical protein	-
EKI55_RS28080	57380..57589	-	210	WP_000866036.1	hypothetical protein	-
EKI55_RS28085	57630..57953	-	324	WP_000427403.1	hypothetical protein	-
EKI55_RS28095	58483..58857	-	375	WP_021500037.1	hypothetical protein	-
EKI55_RS29050	58908..59198	-	291	WP_170995147.1	hypothetical protein	-
EKI55_RS28105	59217..59582	+	366	WP_032345630.1	hypothetical protein	-
EKI55_RS28110	59582..60769	+	1188	WP_000937595.1	IS91 family transposase	-
EKI55_RS28115	60952..61630	-	679	Protein_66	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / toxB / hlyD / hlyB / hlyA / hlyC	1..157534	157534

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9818.23 Da
• Isoelectric Point: 9.5163

>T120460 WP_000702245.1 NZ_CP037942:c57007-56747 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T120460 NZ_CP037942:c57007-56747 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT120460 WP_001132407.1 NZ_CP037942:c56757-56506 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT120460 NZ_CP037942:c56757-56506 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCTGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A6X6XTU7